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摘要:1.Start a 5 ml overnight culture from a frozen stock (in the -70℃)in the following media:For 100 ml of M9CA media tyrp 10 ml of 10X M9 salts5 ml of 10% casamino acids0.2 ml 1M MgSO40.01 ml 1M CaCl20.5[阅读全文]