摘要:1. Add 400 μl of TE buffer then 400 μl of 1-butanol to the oligonucleotide glass vial.2. Vortex well, then spin down in tabletop centrifuge for 10 minutes at ~2,000. rpm¹s.3. Remove top, bu[阅读全文]

摘要:SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g Ureaup to 500 ml Q 250 ml 10X TBE 120.8 g Acrylamideup to 500 ml Q 4.1 g BISup to 500 ml Q2.5 M NH4 OAc19.2 g NH4 OAcup t[阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:This is a question asked quite a bit, most recently (late September, 1995) in the newsgroup. Here are two methods, and who submitted them. UV Shadowing Electrophorese the oligo via PAGE. Place the gel[阅读全文]

摘要:Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligonucleotide will[阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1. Quantitate crude oligo solution via UV spectrophotometry. Assume 1.0 A260 unit is equal to 33μl/ml. Dry down 100μl of ol[阅读全文]