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搜索“TAG:Purification”找到相关内容18篇,用时1.084840秒

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Purification of PCR fragments for cloning
词条创建者:admin创建时间:12-09 23:22
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摘要:After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder of the reaction is concentrated by ethanol precipitation, resuspended in buffer, and subjected to a simultaneous fill-i[阅读全文]

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Purification of DNA from lambda phage
词条创建者:admin创建时间:12-09 23:13
标签: Purification lambda

摘要:This is a modification of the procedure in Short Protocols (1-41,1-45)1.Prepare a 50 ml liquid lysate:A.mix 2xlO8 E.coli cells with 100μl phage (from one picked plaque in 500μl SM),and 100μl [阅读全文]

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Purification of PCR fragments for cloning(PCR克隆片段纯化)
词条创建者:admin创建时间:12-09 22:27
标签: Purification PCR fragments for cloning 克隆片段 纯化

摘要:After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder of the reaction is concentrated by ethanol precipitation, resuspended in buffer, and subjected to a simultaneous fill-i[阅读全文]

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Purification of TFIIIC from yeast
词条创建者:admin创建时间:12-09 22:10
标签: TFIIIC

摘要:SummaryWCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC complex is first purified by flag affinity: TFC4 contains a single copy of the flag epitope.The flag purified TFIIIC is[阅读全文]

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Purification of TFIIA from E. coli
词条创建者:admin创建时间:12-09 22:10
标签: TFIIA E.coli

摘要:Purification of TFIIA from E.coliLinda Warfield,Hahn Lab,2003Volumes given are for 2L of cells for each subunit (Toa1 and Toa2).Toa1 and Toa2 subunits are expressed separately in cultures of OD0.6 for[阅读全文]

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Purification of MLCK ( Myosin Light Chain Kinase )from Rab
词条创建者:admin创建时间:12-09 22:10
标签: MLCK

摘要:点击下载PDF文档[阅读全文]

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Purification of Calmodulin from Bovine Brain从牛脑中分离钙
词条创建者:admin创建时间:12-09 22:10
标签: 牛脑 分离钙

摘要:点击下载PDF文档[阅读全文]

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Purification of GST fusion proteins in E.coli GST融合蛋白纯化(方法四)筛选表达
词条创建者:admin创建时间:12-09 22:10
标签: GST融合蛋白纯化

摘要:Screen of GST-Fusion Protein Expression(pGEX system by Amersham: for check clones for expression of the desired fusion protein prior to large-scale purification)Pick several colonies of E.coli transfo[阅读全文]

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Purification of GST fusion proteins in E.coli GST融合蛋白纯化,方法三,纯化小量
词条创建者:admin创建时间:12-09 22:10
标签: GST融合蛋白纯化

摘要:Small scale fusion protein preparationGrow 5ml culture o/n in TB with amp.Add o/n culture to 50ml of TB amp and grow for 3 hours in 37℃ shaker.Induce culture by adding 20μl of 1M IPTG (final [0.4mM[阅读全文]

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Purification of GST fusion proteins in E.coli GST融合蛋白纯化,方法一
词条创建者:admin创建时间:12-09 22:10
标签: GST融合蛋白纯化

摘要:Making GST fusion proteins:(07/19/03)ver.1Grow up 5ml LB with Amp o/n.Add to 45ml LB with Amp37o shake 2.5 - 3 hrs,till OD600 0.4-0.8Put bottles in room temperature water for 10 min to cool down.Add 1[阅读全文]

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Purification of GST fusion proteins in E.coli GST融合蛋白纯化,方法二
词条创建者:admin创建时间:12-09 22:10
标签: GST融合蛋白纯化

摘要:GST Protein Prep.Ver.21)Grow 50ml of culture in LB or TB antibiotic o/n at 37℃ shaker.2)Dilute culture in LB or TB antibiotic 1:103)Grow 3hrs at 37℃.4)Induce culture by adding 0.4 mM IPTG final concen[阅读全文]

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Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
词条创建者:admin创建时间:12-09 22:10
标签: 蛋白纯化 Ni-NTA-Agarose 6xHis

摘要:Low (“Low”)Imidazole Buffer 0.5L100mM Imidizole 3.4g5% glycerol 25ml 100% glycerol50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9)0.1% Tween-20 0.5M 100% Tween-20500mM NaCl50ml 5M NaCldH2[阅读全文]

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Purification of dnEBNA-1/Soft from E. coli BL21 LysS
词条创建者:admin创建时间:12-09 22:10
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摘要:Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (dnEBNA-1/Soft)in E.coli BL21 LysS per 0.5L LB ampicillin (grow two 0.5L cultures of each)Incubate ~2hrs 37℃250rpm until [阅读全文]

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Purification of recombinant sBRF M166L
词条创建者:admin创建时间:12-09 22:09
标签: Purification recombinant sBRF M166L

摘要:J.Movius,K,Coachman,and S.Hahn (Hahn Lab)last modified 10/9/00Induction of BRF in bacteria and purification on Ni-NTA agaroseTransform BRF plasmid into strain BL21 DE3 (pLysS)or JM25 (DE3 containing t[阅读全文]

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Purification of mAb (IgG)
词条创建者:admin创建时间:12-08 01:11
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摘要:1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight. (2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) (3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfat[阅读全文]

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Purification of Antiserum or Ascites by Protein A/G Chromatography
词条创建者:admin创建时间:12-08 01:11
标签: purification antiserum protein A/G chromatography purification ascites

摘要:1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A or protein G)(2) Ice-cold Tris-buffered saline (TBS[阅读全文]

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Purification of Demethylated Sphingomyelin 纯化脱甲基鞘磷脂
词条创建者:admin创建时间:12-06 23:40
标签: Purification of Demethylated Sphingomyelin 纯化 脱甲基 鞘磷脂

摘要:Contributor: Suprya JayadevDate: Mar. 11, 1991I. Lower, chloroform phase:1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample completely, but sufficiently to yield a f[阅读全文]

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Purification of Gene Targeting Vector DNA for Electroporation
词条创建者:admin创建时间:12-06 23:14
标签: Transgene Mice DNA Preparation

摘要:1. Purify plasmid from bacteria. We recommend the Qiagen EndoFree Plasmid Maxi kit for the purification of the targeting vector plasmid from bacteria. Please follow the directions in the kit. Electrop[阅读全文]

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