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Single Strand DNA Prep. for Sequencing
词条创建者:admin创建时间:12-09 23:24
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摘要:This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly).Solutions2X YT Media16 g tryptone10 g yeast extract5 g NaCl1 ml 1N NaOHup to 1 liter with Q[阅读全文]

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Single Clone Excision Protocol
词条创建者:admin创建时间:12-09 23:13
标签: 单克隆

摘要:1.Pick plaques and put in 500 ml sterile SM with 20 ml chloroform.Store in 4° overnight.2.Be sure we have SOLR and XL1-Blue cells in 10 mM MgSO4,no more than 1 week old.If not,set up a culture of [阅读全文]

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SINGLE STEP PROKARYOTIC RNA ISOLATION
词条创建者:admin创建时间:12-09 22:51
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摘要:In the past, poly(A)+ RNA has not been detected in prokaryotes.In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and pot[阅读全文]

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Single Primer ("Semi-Random") PCR
词条创建者:admin创建时间:12-09 22:27
标签: Single Primer Semi-Random PCR

摘要:DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources of DNA.At sufficiently low stringency, any primer [阅读全文]

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Single tube confirmation PCR protocol
词条创建者:admin创建时间:12-09 22:27
标签: Single tube confirmation PCR protoco

摘要:The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of fi[阅读全文]

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