摘要:This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly).Solutions2X YT Media16 g tryptone10 g yeast extract5 g NaCl1 ml 1N NaOHup to 1 liter with Q[阅读全文]

摘要:1.Pick plaques and put in 500 ml sterile SM with 20 ml chloroform.Store in 4° overnight.2.Be sure we have SOLR and XL1-Blue cells in 10 mM MgSO4,no more than 1 week old.If not,set up a culture of [阅读全文]

摘要:In the past, poly(A)+ RNA has not been detected in prokaryotes.In the early 80s, a new method of isolating RNA from bacteria was developed, involving lysis by protease K in the presence of SDS and pot[阅读全文]

摘要:DescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources of DNA.At sufficiently low stringency, any primer [阅读全文]

摘要:The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformation along with a wild-type control. A series of fi[阅读全文]