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搜索“TAG:Standard”找到相关内容3篇,用时1.134337秒

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Standard?Ligation
词条创建者:admin创建时间:12-09 23:22
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摘要: Prepare a ligation mix:Ligation Mix (2x)10x ligase buffer 1.0 ml digested vector(0.1 mg/ml) 1.0 ml H2 O 6.0 ml total 8.0 ml divide ligation mix into two Eppendorf tubesLigation RxnInsert Control liga[阅读全文]

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Standard?hybridization?technique
词条创建者:admin创建时间:12-09 23:13
标签: hybridization

摘要:The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by he[阅读全文]

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Standard PCR Protocol
词条创建者:admin创建时间:12-09 22:27
标签: Standard PCR Protocol

摘要:Recommended Reagent Concentrations: Primers: 0.2 - 1.0 uM   Nucleotides: 50 - 200 uM EACH dNTP   Dimethyl sulphoxide (DMSO): 0 - 10% (v/v)   Taq polymerase: 0.5 - 1.0 Units/50ul rxn Target DNA: 1 ng -[阅读全文]

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