摘要: Prepare a ligation mix:Ligation Mix (2x)10x ligase buffer 1.0 ml digested vector(0.1 mg/ml) 1.0 ml H2 O 6.0 ml total 8.0 ml divide ligation mix into two Eppendorf tubesLigation RxnInsert Control liga[阅读全文]

摘要:The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by he[阅读全文]

摘要:Recommended Reagent Concentrations: Primers: 0.2 - 1.0 uM 　　Nucleotides: 50 - 200 uM EACH dNTP 　　Dimethyl sulphoxide (DMSO): 0 - 10% (v/v) 　　Taq polymerase: 0.5 - 1.0 Units/50ul rxn Target DNA: 1 ng -[阅读全文]