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搜索“TAG:sequencing”找到相关内容6篇,用时1.055580秒

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Sequencing of BAC DNA--BAC DNA测序
词条创建者:admin创建时间:12-09 23:24
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摘要:16 µl BigDye Terminator Ready Reaction Mix1 µg BAC DNA (determined from gel)10 pmol primerwater to 40 µlHeat tubes at 95℃ for 5 min., then perform 30 cycles of:95℃ x 30 sec55℃ x 10 s[阅读全文]

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SEQUENCING GELS
词条创建者:admin创建时间:12-09 23:21
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摘要: 10X TBE, per literSequencing dye-----------------------------------108g Tris base80% formamide55g boric acid10mM NaOH40ml 0.5M EDTA1mM EDTA0.1% xylene cyanol10% APS0.1% bromophenol blue--------------[阅读全文]

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SEQUENCING GELS
词条创建者:admin创建时间:12-09 23:19
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摘要:10X TBE, per literSequencing dye-----------------------------------108g Tris base80% formamide55g boric acid10mM NaOH40ml 0.5M EDTA1mM EDTA0.1% xylene cyanol10% APS0.1% bromophenol blue---------------[阅读全文]

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Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
词条创建者:admin创建时间:12-09 23:15
标签: Sequencing Reaction

摘要:1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be [阅读全文]

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Sequencing-Template Preparation, DS测序模板准备【Upstate Medical University】
词条创建者:admin创建时间:12-09 23:14
标签: 测序模板

摘要:Amberg Lab ,Upstate Medical University http://www.upstate.edu/biochem/amberg/protocols/seq_temp.html Notes on DNA : The cleaner the better.If mini DNA use phenol extracted and use the entire mini prep[阅读全文]

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Sequencing off Bacterial Genomic DNA with ABI Big Dye Terminators
词条创建者:admin创建时间:12-09 23:12
标签: DNA Sequencing

摘要:The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoF[阅读全文]

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