摘要:Isolation of DNA from Mouse Tail Biopsies 1. Obtain tail biopsies from 2 to 3 week old mice: Hold mouse firmly at base of tail with one hand, with the other cut off 0.5 to 1.0 cm of the tail tip with [阅读全文]

摘要:1.Excise DNA section from gel into eppendorf tube.2.Add 2-3 volumes of NaI (NaI for geneclean - dissolve 89.9 g NaI in 100ml dH2O store in light proof bottle) solution to gel fragment (best to be on t[阅读全文]

摘要:1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is correct for most applications.2. Run out DNA fragments. For fragments greater than 500 bp, run the xylene cyanol dye to [阅读全文]

摘要:Retroelements and their derivatives are a ubiquitous and abundant component of plant genomes. From the 1990s, PCR based techniques have been developed to isolate the elements from genomic DNA of diffe[阅读全文]

摘要:Isolation of DNA from Agarose Gels (Paper Slurry Method)This procedure isolates DNA from agarose gels by filtration through a filter-paper column.The column is made in a 500 µL tube from a slurr[阅读全文]

摘要:RNA for S1 or PE analysis must be free of chromosomal DNA.This can be accomplished by extraction of the RNA with hot phenol, but phenol can, especially if its pH is acidic, cause the specific loss of [阅读全文]

摘要:1.Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2.Sacrifice the pregnant female mouse (day 13 or 14 p.c.) by cervical dislocation. Dissect out the uterine horn[阅读全文]

摘要:完整的实验方法请点击下面的链接下载： 02 Isolation of linearized BAC DNA for microinjection to produce transgenic animals.pdf[阅读全文]

摘要:完整的实验方法请点击下面的链接下载：04 Isolation of YAC DNA for microinjection to produce transgenic animals.pdf[阅读全文]

摘要:1. Obtain tail biopsies from 2 to 3 week old mice: Hold mouse firmly at base of tail with one hand, with the other cut off 0.5 to 1.0 cm of the tail tip with a scalpel or single edge razor blade. 2. A[阅读全文]