摘要:MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or 40% acrylamide-bis (19:1)10X TBEurea10% ammonium persulfateTEMED60 cc. syringeRain-exPreparation of glass plates:Wash an[阅读全文]

摘要:The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988.Inoculate 2-5 ml of L broth containing the appropriate an[阅读全文]

摘要:Preparation of Nested Deletions Procedure:1) Need to cut 10 µg of plasmid in two spots ('A' and 'B') in polylinker. 'A'cut is 'ith a restriction enzyme which gives a 3' recessed end (exonuclease[阅读全文]

摘要:CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:--GENTLENESS is key through step 10!--Try to do prep steps 1-8 at the side of the centrifuge--Make Solution III and plac[阅读全文]

摘要:Author: Long-Cheng Li Source: Protocol Online Abstract: Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing re[阅读全文]

摘要:点击浏览该文件[阅读全文]

摘要:Preparation of Sonicated Salmon Sperm DNA Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps, weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris, pH 7.6. Allo[阅读全文]

摘要:Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation procedure. ABI; 1995MaterialsGTE buffer (50mM glucose, 25mM Tris-HCl (pH8.0), 10mM EDTA (pH 8.0)) (200µl/tube) 0.2N [阅读全文]

摘要:1.Inoculate a couple of 20 ml LB broth in a 125 ml flasks with 0.5 ml of an overnight culture of the recipient strain. 2.Shake at 37 ℃ until O.D. 600=0.13 - 0.15 (1 - 2 hours). Measure by taking some [阅读全文]

摘要:These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subse[阅读全文]

摘要:Donis-Keller Lab Manual, Department of Genetics in Washington University School of Medicine http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid13.html Purpose:To maintain lab stock of highly efficient l[阅读全文]

摘要:The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988.1.Inoculate 2-5 ml of L broth containing the appropriate [阅读全文]

摘要:Denatured Salmon Sperm DNA is used in prehybridization and hybridization solutions to reduce background signal.The Salmon Sperm DNA (Sigma No.D-1626)is first "denatured" in large volumes and then agai[阅读全文]

摘要:Preparation of linear polyacrylamide used as carrier in ethanol precipitation of nucleic acidsLinear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with eth[阅读全文]

摘要:PurposeThe protocol describes how to prepare human tonsil lysate for use in purification of ICAM-1,LFA-1,and PNAd.MaterialsSafety EquipmentsLab CoatLatex GlovesFace MaskBench PaperFresh human tonsil t[阅读全文]

摘要:1) Harvest 1x105 to 108 cells. Wash 1x w/PBS and freeze in liquid N2 and store @ -70℃ 2) Resuspend each pellet in 1.5ml lysis buffer (300μl 5x Lysis, 75μl 200mM VR, 1.125ml H2 O3) Incubate on ic[阅读全文]

摘要:Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 3000?5000 x g using a swing-out[阅读全文]

摘要:Preparation of G+A Marker　　Author: Long-Cheng Li　　Source: Protocol Online　　Abstract: Simplified method for preparing G+A ladder run along with footprinting reaction. It's much 　　　　　　　simple than the o[阅读全文]

摘要:Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedures should be done at 4ºC using precooled reagents. For rat samples, immediately remove brain from the cranium into[阅读全文]

摘要:1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration).2.Read OD205,OD280 (using 0.1M MOPS buffer as blank)3.Affigel-10 is stored frozen at < -70℃.Thaw at 4℃[阅读全文]