摘要:NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR. (from Ruixia, 7/99, from protocol by Stef Oehen 7/94). 1. Put 1 cm tail in 1.5 ml microcentrifuge tube.2. Add 500µl Tail Buffer an[阅读全文]

摘要:About 1/2 to 1 cm of tail should be cut from properly marked mice (using toe or ear clipping, etc), about the age of 2-3 weeks. Place these tails into marked 1.5 ml Eppindorf tubes for processing; the[阅读全文]

摘要:DNA isolation for Southern analysis 完整的实验方法请点击下面的链接下载：10Tail Prep for Southern Blots.pdf[阅读全文]

摘要:1. Obtain the last 2mm of tail and place directly into 200ul 1X PCR Buffer with Nonionic Detergents (PBND) in a 1.5ml microfuge tube. (Tails can be stored at frozen in PBS or PBND until use.) 2. Add 1[阅读全文]

摘要:NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR. (from Ruixia, 7/99, from protocol by Stef Oehen 7/94). 1. Put 1 cm tail in 1.5 ml microcentrifuge tube. 2. Add 500µl Tail Buffer a[阅读全文]