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摘要:The gel shift, or electrophoretic mobility shift, assay provides a simple and rapid method for detecting DNA -binding proteins. This method has been used widely in the study of sequence-specific DNA -[阅读全文]
摘要:John Mundy, Institute of Molecular Biology, Copenhagen, Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#Gelshift 1)Nuclear extract: see nucprep.ptc & nucext.ptc extracts should be at leas[阅读全文]
摘要:Mirmira Laboratory at the University of VirginiaThere are multiple variations to this protocol, but we find that this one works well in all cases we tested.http://faculty.virginia.edu/mirmira/resource[阅读全文]
摘要:What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we are separating molecules of DNA that we got from [阅读全文]