摘要:does anyone have a reasonably successful protocol for isolating dna from bones---bones that are highly compromised in integrity---i.e.,really bad shape?I've tried and am well seasoned in the tradition[阅读全文]

摘要:Smith Lab,Division of Biological Sciences,University of Missouri http://www.biosci.missouri.edu/smithgp/PhageDisplayWebsite/AmplifyingLibrary.docThis section describes how to amplify a library with mi[阅读全文]

摘要:This section describes how to amplify a library with minimal danger of substantially reducing its diversity.Amplification is accomplished by infecting fresh cells with a portion of the library,growing[阅读全文]

摘要:1.Two rounds of DNA synthesis to incorporate fixed sequencesPrepare the following:Reaction tube containing the following:7ml DNA to be amplified and labelled2ml 5X T7 Sequenase Buffer1ml 50mM Pimer 1 [阅读全文]

摘要:1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml .2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 [阅读全文]