摘要:For every 4 mls of culture, dissolve the BAC DNA pellet in 40 µl of water. for example: Usually each BAC is grown in 20 mls LB/CM total, then is dispensed into one Autogen tube (4 mls in each of[阅读全文]

摘要:1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl[阅读全文]

摘要: 1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB with 20µg/ml chloramphenicol and cultured at 37°C with shaking for 16hrs. The liquid bacterial culture is tra[阅读全文]

摘要: Hi everybodyI am having a problem in the lysis of a moderatelly halophilic strain that I am using to prepare DNA. It is Gram negative strain but it resists all kind of things that I tried, any help w[阅读全文]

摘要:BAC提取前准备（前两天 ）配制含25 mg/l Kan的LB培养基选择平板，在超净工作台上用接种环从购买的BAC划线接种至选择平板，于37℃培养箱培养过夜，供选择单。BAC提取前准备（前一天）1、在超净工作台上，用50 µg/ml Kan和[阅读全文]

摘要:Background Information of RNA iRNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing.[阅读全文]

摘要:1)Place gene of interest between T7 promoters in Òdouble T7Ó plamsid.(slightly more difficμlt alternative:Place gene of interest in hairpin/inverted repeat configuration behind T7 pro[阅读全文]

摘要: Objective:This protocol allows rapid detection of transformation success when primers are available to allow determination of correct ligation products by size or hybridization. Procedures:Start => [阅读全文]

摘要:Materials Expression plasmid (e.g.,pET/Ncd in BL21(DE3)pLysS host cells)ZB = 10 g NZ-Amine A5 g NaCl860 ml final volume10X M9 Salts = 1 g NH4Cl3 g KH2PO46 g Na2HPO4100 ml final volumeM9ZB = 860 ml ZB [阅读全文]