摘要:1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Transfer 1.5 mls of culture to eppendorf tube.Spin for 1 minute on high and remove supernatant.3、Add 700 µl STET. Add [阅读全文]

摘要:Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 m[阅读全文]