摘要:Costen 氏综合征又称颞颌关节综合征；颞颌关节疼痛一切能紊乱综合征。 【病因和机理】 臼齿缺如、不正咬合或复咬合导致咬肌过度收缩或下颌关节变性，下颌踝移位，下颌神经之耳颞支及鼓索支承受机械性刺激，[阅读全文]

摘要:Collet –Sicard氏综合征又称枕骨髁—颈静脉孔连接部综合征；腮腺后窝综合征；半侧舌喉肩咽瘫综合征。 【病因和机理】 因肿瘤、血管病变、外伤、炎症等病变由颈静脉孔向枕骨前髁管扩延致一侧性舌咽、[阅读全文]

摘要:Cockayne氏综合征又称小头、纹状体小脑钙化和白质营养不良综合征；侏儒症、视网膜萎缩和耳聋综合征。 【病因和机理】 病因未明，可能与常染色体隐性遗传有关。 【临床表现】 以早老为其特征，婴儿期正常[阅读全文]

摘要:【临床表现】 患者感到自己已不复存在，或是一个没有五脏六腑的空虚躯壳，并认为其他的人，甚至整个世界包括房子、树木都不存在了。 【鉴别诊断】 （一）抑郁症（depression）　 患者情绪低落，思维缓[阅读全文]

摘要:Protocol For the original protocol describing CGH see the article by Kallioniemi et al . For an example of how this technique has been utilized by the NCI Prostate Cancer Working Group see the section[阅读全文]

摘要:METHOD:1.Isolate chromosomal DNA by using the CTAB protocols in current Pr M R.2.Make a CsCl preparation of pLAFr3 (or other appropriate cosmid).3.Cut pLAFr with BamHI phenol ext. + ETOH preparation.4[阅读全文]

摘要:This method is after Marchuk,D.,et al.,1991,Nucl.Acids Res.19(5),pp1154. You will need:10 x Taq buffer (Promega)Taq Polymerase (Promega)Phenol/chloroform mix100mM dTTPTE bufferAbsolute ethanol70% etha[阅读全文]

摘要:1) The ligation mixture contains the following:vector (~100 ng)　　insert (equimolar or 2 or 3 X molar concentration of vector)　　water added to 18 µl2) Heat the mixture at 45 ℃ for 5 min. to melt [阅读全文]

摘要:(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu)Typically, 2.5 - 3 volumes of an ethanol/acetate solution is added [阅读全文]

摘要:CONCERT细胞质RNA 纯化试剂是一种新颖的单相酚溶液，是专门设计用来从新鲜或冷冻的培养的动物细胞 或组织中快速、简单地纯化高质量的细胞质RNA 。应用利用CONCERT™细胞 质RNA 试剂分离的总RNA 能够用于构建cDNA文[阅读全文]

摘要:This procedure will work for both yeast and E. coli:Take a small colony and suspend it in 5ul of H2O in a PCR tube. Heat for 5 min at 95℃ and then spin the condensation down in a microfuge. Set up the[阅读全文]

摘要:A method to re-PCR unique bands from products of mixed sizeINTRODUCTIONThe products of a PCR reaction - especially when this is done on eukaryotic genomic DNA, and when using degenerate primers - ofte[阅读全文]

摘要:Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.2720mM Hepes pH 7.90.1% NP400.2mM EDTA20% glycerolLysis Buffer: 0.75M KClNeed buffers containing 0,0.1M,0.6M & 1.0M KCL in addition to 0.3M,1.5[阅读全文]

摘要:Adapted from W.C.Copeland,Protein Exp.& Purif 9,(1997)1-9 and Schneider,et.al.,JBC 273:34 (1998)21608-21615.Obtained from Fanning lab and revised by LSM,7/23/01Obtained cell stock of BL21(DE3)cells co[阅读全文]

摘要:day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C, shaking. 3. Prepare: ~ 3.5 L sterile H2O chil[阅读全文]

摘要:一、仪器开/关机程序1、开机程序接通电源，依次打开打印机、显示器、电脑主机和仪器开关，仪器自动进行一次自检。2、关机程序依次切断仪器、电脑主机、显示器和打印机开关。二、分析仪系统设置程序1、分析程序框架（Protocol Confi[阅读全文]

摘要:《Confocal Laser Scanning Microscope Instruction》下载点击这里下载[阅读全文]

摘要:点击浏览该文件 [阅读全文]

摘要:The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recomb[阅读全文]

摘要:1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination).2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller).3. wash the beads[阅读全文]