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摘要:Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.2720mM Hepes pH 7.90.1% NP400.2mM EDTA20% glycerolLysis Buffer: 0.75M KClNeed buffers containing 0,0.1M,0.6M & 1.0M KCL in addition to 0.3M,1.5[阅读全文]
摘要:Contributor: Suprya JayadevDate: Mar. 11, 1991Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform.2) Place a small portion of glass wool at the base of the column.3[阅读全文]