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摘要:day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr, Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C, shaking. 3. Prepare: ~ 3.5 L sterile H2O chil[阅读全文]