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摘要:This method is after Marchuk,D.,et al.,1991,Nucl.Acids Res.19(5),pp1154. You will need:10 x Taq buffer (Promega)Taq Polymerase (Promega)Phenol/chloroform mix100mM dTTPTE bufferAbsolute ethanol70% etha[阅读全文]