摘要:1．固相载体的选择  载体的种类很多，其中包括纤维素、交联右旋糖酐、葡萄球菌聚苯乙烯、聚丙烯酰胺等。从使用形式上可有凹孔平板、试管、珠粒等。 聚苯乙烯凹孔板是应用最广泛的一种载体。聚[阅读全文]

摘要:elisa实验方法操作标准，用于ELISA测定的临床标本最为常用的是血清(浆)，有时因为特定的检测目的，也用到唾液、脑脊液、尿液、粪便等标本。目前临床上使用血清标本测定的标志物一般有传染性病原体的抗原和[阅读全文]

摘要:Eaton-Lambert氏综合征又称类重症肌无力综合征；肌无力—肌病综合征；癌肿伴肌无力综合征。 【病因和机理】 多数伴发于癌肿，以小细胞型肺癌最多见。由于运动神经末稍乙酰胆碱的释放减少，使神经肌肉[阅读全文]

摘要:EB病毒（Epstein-Barr virus,EBV）是Epstein和Barr于1964年首次成功地将Burkitt非洲儿童淋巴瘤细胞通过体外悬浮培养而建株，并在建株细胞涂片中用电镜观察到疱疹病毒[阅读全文]

摘要:Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate thi[阅读全文]

摘要:This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.Materials 3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃). Cold 100% Etha[阅读全文]

摘要: 1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract and NaOAc/EtOH ppt. 2. Prepare one S1 nuclease tube (on ice) for each time point: 15ml S1 Buffer + 0.25U S1/ml (5U). 3. Use 5ml [阅读全文]

摘要:Subcloning should be easy and fast, and work every time. The following protocols minimize the number of manipulations required to prepare DNA fragments for ligations, thereby both saving time and incr[阅读全文]

摘要:Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final co[阅读全文]

摘要:Solutions Procedure Design complementary oligonucleotides with compatible half sites on the ends (I use BamHI and BglII). Dry down 300 ng of the two purified oligos together and resuspend in 9 m l Q w[阅读全文]

摘要: 1、Put a 0.5-1.5 CM MOUSE TAIL SNIP in a 1.5 ml eppindorf tube and store frozen until digestion.Make certain that the eppindorf tubes seal well,otherwise your samples may leak out during the shaking s[阅读全文]

摘要:20cm Gel Preparation, Sample Separation, and Visualization:　　Glass Plate Preparation:[阅读全文]

摘要:The Erase-a-Base® System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA . The system is based on the procedu[阅读全文]

摘要:This clearly is an "old procedure revisited" but is extremely efficient because 1) the amount of resulting nested fragment set is at least 5 to 10 times more than obtained by Sephadex G-50 filtration [阅读全文]

摘要:1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate (about one week at 21°).2. Macerate two plates of mycelia in a blender with 50 ml of .002 P liquid medium. Inocula[阅读全文]

摘要:Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 mls0.5 M EDTA 0.24 mls1 M NaCl 3.0 mlsqs to 600 mls dH2OAcetate cushion 3 M NaAcetate pH 4.8 480 μl0.1 % Bromphenol Blu[阅读全文]

摘要:Kitto Lab, The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/ethanolPpt.html This procedure allows the concentration of DNA samples from dilute[阅读全文]

摘要:QUOTEjulianne: been using sod acetate- isoprop precipiation with no problems all the while.QUOTETurtle: my suggestion would be to ethanol precipitate,QUOTEfor PCR product purification used for cloning[阅读全文]

摘要:Sample Preparation Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brush[阅读全文]

摘要:PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel, funneled down into the v-shaped channel and trapped within a high salt cushion resting at the bottom of th[阅读全文]