摘要:This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.Materials 3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃). Cold 100% Etha[阅读全文]

摘要:Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure the volume of the DNA sample. Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final co[阅读全文]

摘要:This clearly is an "old procedure revisited" but is extremely efficient because 1) the amount of resulting nested fragment set is at least 5 to 10 times more than obtained by Sephadex G-50 filtration [阅读全文]

摘要:Kitto Lab, The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/ethanolPpt.html This procedure allows the concentration of DNA samples from dilute[阅读全文]

摘要:QUOTEjulianne: been using sod acetate- isoprop precipiation with no problems all the while.QUOTETurtle: my suggestion would be to ethanol precipitate,QUOTEfor PCR product purification used for cloning[阅读全文]

摘要:This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.Materials•3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃)•Cold [阅读全文]