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搜索“TAG:FOOTPRINTING”找到相关内容4篇,用时0.011016秒
摘要:FootprintingFootprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. Examples: hormone-receptor complexes that bind to their hormone response el[阅读全文]
摘要:1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtOH ppt which can enature dsDNA 2)probe mix/rxn: volumes x # samples 1μl probe (0.1?0.5ng or 10?20kcpm) 0.15μl 20m[阅读全文]
摘要:John Mundy ,Institute of Molecular Biology,Copenhagen,Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#Footprint 1)probe: same as used for gelshift),isolated by isotacelectrophoresis w/out[阅读全文]
摘要: DNase I Footprinting assay is a method of studying DNA -protein interaction and identifying the DNA-sequence to which a protein binds. First, a target DNA-fragment about 100-300 bp in length is eithe[阅读全文]