摘要:Guillain–Barre氏综合征又称，急性感染性多发性神经炎；急性多发性神经根炎；急性上升性麻痹；Landry麻痹。 【病因和机理】 病因至今尚不明确，多数学者倾向于慢病毒感染与自体免疫学说，推测[阅读全文]

摘要:Gerstmann氏综合征又称，左侧角回综合征；优势半球综合征。 【病因和机理】 主侧半球顶叶与颞叶角回病变，以顶后动脉或角回动脉闭塞常见。亦可见于脑瘤、脑外伤、脑萎缩、酒精中毒、一氧化碳中毒、各种精[阅读全文]

摘要:Garcin氏综合征又称，一侧颅底综合征；Garcin-Guillain 氏综合征。 【病因和机理】 颅底肿瘤（蝶骨、颞骨、眶内、中颅窝脑膜瘤、岩骨附近肉瘤）或来自颅骨下部的颅外肿瘤（上颌窦、鼻腔、鼻[阅读全文]

摘要:GBS又名急性特发性多神经炎、Landry-Guillain-Barré上升性麻痹或综合征，过去曾称之为脊髓神经根多发性神经元炎、神经根神经元炎、感染性多发性神经炎。GBS是病因未明的综合征，可能为某[阅读全文]

摘要:We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hybridization. The genomic DNA was fragmented to 500 to 1000bps before labeling. The following protocol should produce eno[阅读全文]

摘要:1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled with LB agar, stabbed with a toothpick which has been put into a bacterial colony). These are then frozen; at -70℃ they wil[阅读全文]

摘要:The following guidelines should be taken into account when designing modified oligonucleotides. 1.Sequence Length - SYNTHEGEN can synthesize oligonucleotides from 5 to 110 bases in length. Most sequen[阅读全文]

摘要:macerate tissue in Eppendorf tube without butter at RT add 400 m l extraction buffer vortex for 4 sec leave sample at RT until other samples are ready (> 1 h) spin in microfuge for 1 min transfer 300 [阅读全文]

摘要:1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at rosette stage if possible). Use 96 well plates (1 or 2 ml, E&K, po[阅读全文]

摘要:用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.0。3、模板DNA 不要被污染。4、准确定量，不要少于3μg。[阅读全文]

摘要:Publisher: Bios Scientific Publishers LtdNumber Of Pages: 608Publication Date: 2002-06-15Sales Rank: 4476658ISBN / ASIN: 1859962289EAN: 9781859962282Binding: HardcoverManufacturer: Bios Scientific Pub[阅读全文]

摘要:点击浏览该文件[阅读全文]

摘要: Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie, 1979 ([阅读全文]

摘要:The gel shift, or electrophoretic mobility shift, assay provides a simple and rapid method for detecting DNA -binding proteins. This method has been used widely in the study of sequence-specific DNA -[阅读全文]

摘要:Sample DNAs should not be highly degraded nor contain PCR inhibitors, such as high concentrations of heme or chelating agents. For each individual assayed, 250 ng of genomic DNA are digested separatel[阅读全文]

摘要:1) Digest DNA with two restriction enzymes such that one end is ExoIII susceptible (blunt or 5' overhang) and the other is ExoIII resistant (5' recessed). The ExoIII susceptible end should be such tha[阅读全文]

摘要:GenomicPlantDNARestrictionDigestionForSoutherns.pdf[阅读全文]

摘要:John Mundy, Institute of Molecular Biology, Copenhagen, Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#Gelshift 1)Nuclear extract: see nucprep.ptc & nucext.ptc extracts should be at leas[阅读全文]

摘要:Gene cloning or recombinant DNA technology is the joining of two or more segments of DNA to generate a single DNA molecule capable of autonomous replication within a given host (1). Ligase enzymes cat[阅读全文]

摘要: macerate tissue in Eppendorf tube without butter at RT add 400 ml extraction buffer vortex for 4 sec leave sample at RT until other samples are ready (> 1 h) spin in microfuge for 1 min transfer 300 [阅读全文]