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摘要:SolutionsElution Buffer50 mM Tris 7.5 5 ml 1M Tris pH 7.50.1% SDS 0.1 g SDS0.1 mg/ml BSA 1.0 mg BSA0.25 mM EDTA 50 ml 0.5M EDTA2.5% glycerol 2.5 ml glycerolup to 100 ml with QFor every 10 ml add:10 ml[阅读全文]