摘要:Ig分为IgM、IgD、IgA、IgD和IgE五类。各类Ig的不同功能主要与其结构有关。机体内许多细胞表面具有不同类IgFe的受体，通过Fe受体与IgFe的结合，参与Ig介导的生理功能或病理损伤过程。[阅读全文]

摘要:ECKDescriptionThis method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley, 1985). It is a useful way to avoid coprecipitation of proteins and accumulation of salt in the fin[阅读全文]

摘要:Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk• NaI solution• New WashProcedure:1) Run digested DNA out on agarose gel slowly (70 V on BioRad gel)2) Use lon[阅读全文]

摘要:Mutagenesis is a fundamentally important DNA technology which seeks to change the base sequence of DNA and test its effect on gene or DNA function. The mutagenesis can be conducted in vivo (in studies[阅读全文]

摘要:Isolation of DNA from Mouse Tail Biopsies 1. Obtain tail biopsies from 2 to 3 week old mice: Hold mouse firmly at base of tail with one hand, with the other cut off 0.5 to 1.0 cm of the tail tip with [阅读全文]

摘要: Hi,does anyone know what is the best temperature and time for Isoprop precipitation of DNA? I usually do at room temperature and centrifuge immediately. Can I improve this? And would it matter if I k[阅读全文]

摘要:I am working with very small amount human tissue. Is there anyone who had the experience of using glycogen with 100% ethanol in pheno/chloroform DNA extraction protocol ? Does it realy work to increas[阅读全文]

摘要:1.Excise DNA section from gel into eppendorf tube.2.Add 2-3 volumes of NaI (NaI for geneclean - dissolve 89.9 g NaI in 100ml dH2O store in light proof bottle) solution to gel fragment (best to be on t[阅读全文]

摘要:1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is correct for most applications.2. Run out DNA fragments. For fragments greater than 500 bp, run the xylene cyanol dye to [阅读全文]

摘要:DescriptionThis method was suggested in a BRL "Focus" article several years ago (Zeugin & Hartley, 1985). It is a useful way to avoid coprecipitation of proteins and accumulation of salt in the final [阅读全文]

摘要:In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosineCross-references are to Schwarzacher & Heslop-Harrison 2000. Practical in situ Hybridization. Bios, Oxford[阅读全文]

摘要:Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk• NaI solution• New WashProcedure:1)Run digested DNA out on agarose gel slowly (70 V on BioRad gel)2)Use long [阅读全文]

摘要:Retroelements and their derivatives are a ubiquitous and abundant component of plant genomes. From the 1990s, PCR based techniques have been developed to isolate the elements from genomic DNA of diffe[阅读全文]

摘要:Isolation of DNA from Agarose Gels (Paper Slurry Method)This procedure isolates DNA from agarose gels by filtration through a filter-paper column.The column is made in a 500 µL tube from a slurr[阅读全文]

摘要:实验步骤： 1、Inoculate from an overnight grown in LB.从培养过夜的LB平板上挑取单菌落 。2、Grow in 250 ml "SOB" at 18℃ until OD600 = 0.6.(0.3)接种于250ml SOB，18度培养至OD＝0.6。3、On ice for 10 minutes.菌液置[阅读全文]

摘要:Steve HahnLast Modified Fri,Apr 25,2003Wear gloves throughout,use RNAse free solutions (either autoclaved or sterile filtered)and clean bench and pipetmen with 95% ethanol before use to eliminate any [阅读全文]

摘要:Hey,I am analyzing the methylation status of two regions: one ecompasses 500bp, the other is around 800bp. Could I just designed primers at flanking sites and amply these two regions? I found usually [阅读全文]

摘要:Ⅰ.Isolation of Single Stranded DNA1.Transform plasmid to be mutagenised into JM109. JM109 is endA1, recA1, gyrA96, thi, hsdR17(rk-, mk+), relA1, supE44, l-, d(lac-proAB), [F', traD36, proA+B+, lacIqdM[阅读全文]

摘要:ICP的工作原理：感耦等离子体原子发射光谱分析是以射频发生器提供的高频能量加到感应耦合线圈上，并将等离子炬管置于该线圈中心，因而在炬管中产生高频电磁场，用微电火花引燃，使通入炬管中的氩气电离，产生电子和离子而[阅读全文]

摘要:【目的与要求】了解IVC系统和超净工作台的原理结构、维护、操作程序和注意事项。【仪器与材料】IVC、超净工作台、一次性手套、口罩。【步骤】一、师讲述IVC系统和超净工作台的原理结构、维护、操作程序和注意事项。　　二、学生在教师指[阅读全文]