摘要:Hancock Laboratory Methods.Department of Microbiology and Immunology,University of British Columbia,British Columbia,Canadahttp://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=28MATERIALS:Running buffe[阅读全文]

摘要:1)In the cold room,wash cells with cold "Tris" (the same stuff used for TC).Spin down the cells if you are working with suspension cells in a low speed swinging bucket centrifuge,resuspend in approxim[阅读全文]

摘要:1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off PBS.Wash non-adherent cells in PBS and centrifuge at 800 to 1000 rpm in a table-top centrifuge for 5 minutes to pellet [阅读全文]