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摘要:This protocol describes a method for isolating DNA from plant tissue.Procedure1. Preheat the CTAB Isolation Buffer at 60°C.2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a ch[阅读全文]
摘要:Hi, I am a Biochem student. I am extracting DNA from plants that have been exposed to contaminants, then using restriction enzymes to see if there is any change. I was wondering if there was a differe[阅读全文]
摘要:OverviewProcedure 1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fresh, leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle.3. Scrape the powder into a chilled 50 ml[阅读全文]