摘要: Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector. Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as a linearized vector digested with BamH I and EcoR I. N[阅读全文]

摘要:RNAi相关文章（for free）Prospects of RNA interference therapy for cancerS I Pai, Y-Y Lin, B Macaes, A Meneshian, C-F Hung and T-C WuGene Ther 13: 464-477; advance online publication, December 8, 2005; d[阅读全文]

摘要:1.相似性序列发生Cross－react引起脱靶效应。2.监测手段：对脱靶效应的监测一般通过基因芯片来监测。Gene expression profiling对脱靶效应。3.脱靶效应的特性：转录物表达模式有序列特异性和靶特异性两种。4.大部分脱靶效应[阅读全文]

摘要:Post-transcriptional gene silencing (PTGS), which was initially considered a bizarre phenomenon limited to petunias and a few other plant species, is now one of the hottest topics in molecular biology[阅读全文]

摘要:How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effector step. a | In the first step, input double-strand[阅读全文]

摘要:RNA干扰(RNAi)是由双链RNA(doublestrandsRNA，dsRNA)引起的，广泛存在于动物植物中的序列特异性转录后基因沉默过程，是生物体在进化过程中，抵御病毒感染及由于重复序列和突变引起基因组不稳定性的保护机制。Elbashir等[1[阅读全文]

摘要:关于RNAi整理贴（基因沉默撰写贴）1.RNAi对照解析贴http://www.biox.cn/bbs/post/view?bid=64&id=7151795&sty=3 http://www.biox.cn/bbs/post/view?bid=64&id=7461187&tpg=1&ppg=1&sty=1&age=0#7461187 http://www.bio[阅读全文]

摘要:[阅读全文]

摘要:How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effector step. a | In the first step, input double-strand[阅读全文]

摘要:RNA i Glossary Dicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNA s. Dicer processes long dsRNA into siRNA of 21-23 nt. Interferon - A small an[阅读全文]

摘要:RNAi 实验中双链短RNA（dsRNA）制备过程，本实验方法来自于加州大学Jim教授实验，很权威！Procedure for the Generation of dsRNA for use in RNAi1. Design polymerase chain reaction (PCR ) primers that will amplify[阅读全文]

摘要:RNA沉默是广泛存在于生物中的一种古老现象，是生物抵抗异常DNA的一种保护机制,同时在生物生长发育过程中扮演着基因表达调控的角色。文章对RNA沉默研究中的几个热点问题进行了介绍，按RNA沉默持续时间的不同对其在植物中[阅读全文]

摘要:近年来的研究表明，将与mRNA对应的正义RNA和反义RNA组成的双链RNA(dsRNA)导入细胞 ，可以使mRNA发生特异性的降解，导致其相应的基因沉默。这种转录后基因沉默机制(post-transcriptional gene silencing, PTGS)被称为RNA[阅读全文]

摘要:在植物、昆虫、原生动物体内都发现了自然存在的RNAi现象，实验表明通过目的基因序列的双链RNA可以诱导产生基因沉默现象。要制备较长的dsRNA，最简单的方法就是体外转录合成双向的RNA。如果研究对象不是哺乳动物细胞，那要开[阅读全文]

摘要:RNA interference (RNAi) is a biological process in which the introduction of double-stranded RNA (dsRNA) into a cell results in targeted post-transcriptional gene silencing.Historically, RNAi has been[阅读全文]

摘要:检测基因特异阻断的最常用的方法是进行westernblot分析，比较导入siRNA 前后蛋白表达水平的变化。在一些情况下可能使用检测报告子基因的报告子系统例如β-半乳糖苷酶。也可以应用实时PCR（例如通过使用LUX™引[阅读全文]

摘要:Rnai最近由于RNA 干扰（RNA interference，RNA i）的发现使反义领域的研究增多。这种自然发生的现象最早是在秀丽线虫中发现的（1），是序列特异性地使转录后的基因沉默的有力机制。由于最近两年在RNA i领域取得的进步，已经有[阅读全文]

摘要:The protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates, just scale up or down.Adopted from Clemens[阅读全文]

摘要:基因敲除和转基因动物是功能缺失表型研究的主要方法。然而，获得这些动物是一个漫长而且费用昂贵的过程，同时很多基因的缺失会导致胚胎致死表型，使得基因敲除变得不再可能。灭活蛋白功能的方法包括结构域阴性突变构建和[阅读全文]

摘要:Long double-stranded RNAs (dsRNAs; typically >200 nt) can be used to silence the expression of target genes in a variety of organisms and cell types (e.g., worms, fruit flies, and plants). Upon introd[阅读全文]