摘要:1.Rinse gel on glass plate briefly with distilled water.2.Wash gel in fresh 50% ETOH-12% HAc,with shaking,3 times,1 hour each.(Fix IEF gel ON in 10% TCA).3.Wash gel four times with 10% ETOH-5% HAc for[阅读全文]

摘要:1.Fix gel in 50% methanol for greater than 1 hour rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour.2.Stain gel in 100 mls of stain solution for 30 minutes 3.Rinse gel in [阅读全文]

摘要:1. Rinse gel on glass plate briefly with distilled water.2. Wash gel in fresh 50% ETOH-12% HAc, with shaking, 3 times, 1 hour each. (Fix IEF gel ON in 10% TCA).3. Wash gel four times with 10% ETOH-5% [阅读全文]