摘要: Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992)Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8%[阅读全文]

摘要:Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_transf_rapid.html [阅读全文]

摘要:Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/dmso_yeast_transform.html[阅读全文]

摘要:Mirmira Laboratory at the University of Virginiahttp://faculty.virginia.edu/mirmira/resources_files/Protocols/YeastTransformation 2000.htm[阅读全文]

摘要:Hahn Lab (adapted from J.Leatherwood,Ptashne lab)last modified Mon,Aug 27,2001Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is ad[阅读全文]

摘要:YEAST TWO-HYBRID SCREEN WITH LIBRARY AND BAIT(The following protocol is for use with the LIBRARY transformation only)Day 1:Grow an overnight culture of a single colony of yeast transformed with bait v[阅读全文]

摘要:(The following protocol is for use with the LIBRARY transformation only)Day 1:Grow an overnight culture of a single colony of yeast transformed with bait vector in 2.5 ml of SD-Trp mediumDay 21.The fo[阅读全文]

摘要:Adapted from Yaffe,M.P.and Schatz,G.,PNAS,1984,81,4819-4823.1.Grow 25mls yeast cells to 5x10E6.2.Sequentially spin down cells in a 15 ml polypro tube.3.Wash cells with 1ml ice cold ddH2O,transferrring[阅读全文]

摘要:Horvath and Riezman,Yeast,1994; Gottschling Lab[阅读全文]

摘要:1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.2.Place in incubator at 37°C for 20 minutes. 3.Heat to 90-100°C for 5 minutes.4.Cool on ice. 5.Pour into centrifuge bottles and centrifug[阅读全文]