摘要:Analys of Genomic DNA by Southern Hybridization (Southern Blot) Outline: Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Souther[阅读全文]

摘要:Analysis of Genomic DNA by Southern HybridizationSelect several independent BAC clones containing the same inserts that will be used as a probe. Confirm the BAC clone integrity using by comparing Hind[阅读全文]

摘要:To efficiently determine the chromosomal location of phenotypic mutants, we designed a genome-wide mapping strategy that can be used in any crop for which a dense AFLP (Amplified Fragment Length Polym[阅读全文]

摘要:Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Southern (1975). Genomic DNA is digested with one or more restriction enzymes, a[阅读全文]

摘要:Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2. Allow the silica to settle for 2h.3. Discard the supernatant containing fine particulate matter.4. Repeat steps 2 and 3[阅读全文]

摘要:Analysis of genome-wide association data[阅读全文]

摘要:Hahn Lablast modified 9/30/981. In microfuge tubes, spin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells. Remove YT (or LB) media with a pipetman or drawn out [阅读全文]

摘要:一般性预防1.粘度计的操作环境2.最佳的操作环境：25℃±2℃，湿度45～60%，无尘、无阳光直射和不受加热器或空调影响的地方。3.因粘度计使用音叉振动法，因此，应避免外部的震动，使用稳固的工作台面4.保护内部免受液体的[阅读全文]

摘要:annexinＶ-FITC(FL1 通道)+ PI（FL2通道）双染细胞，FITC阳性＋PI阴性细胞百％就是早期凋亡％。一、原理磷脂酰丝氨酸（Phosphatidylserine，PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，[阅读全文]

摘要:This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.1. Solutions(1) Affigel Blue Prewash0.1 M acetic acid 5.7 ml glacial acetic acid1.4 M NaCl 81 g [阅读全文]

摘要:Most biological systems can be viewed in terms of interactions between receptors and epitopes. Specificity of the interaction between the receptor and epitope is determined by the amino acid sequence [阅读全文]