摘要:We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hybridization. The genomic DNA was fragmented to 500 to 1000bps before labeling. The following protocol should produce eno[阅读全文]

摘要:macerate tissue in Eppendorf tube without butter at RT add 400 m l extraction buffer vortex for 4 sec leave sample at RT until other samples are ready (> 1 h) spin in microfuge for 1 min transfer 300 [阅读全文]

摘要:1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at rosette stage if possible). Use 96 well plates (1 or 2 ml, E&K, po[阅读全文]

摘要:点击浏览该文件[阅读全文]

摘要:GenomicPlantDNARestrictionDigestionForSoutherns.pdf[阅读全文]

摘要: macerate tissue in Eppendorf tube without butter at RT add 400 ml extraction buffer vortex for 4 sec leave sample at RT until other samples are ready (> 1 h) spin in microfuge for 1 min transfer 300 [阅读全文]

摘要:SolutionsProtocol: Digest 5-10 μg genomic DNA overnight with restriction enzyme of choice.Run digested gDNA on 0.8% TAE gel with marker (with no ethidium bromide).Transfer Setup: 1. Remove gel and [阅读全文]

摘要:I am trying to digest mouse genomic DNA for a southern blot. When digested with either HIndIII or NdeI, I get a nice smear as expected. But when I digest with either speI or BglII (neither one of whic[阅读全文]

摘要:1.grow plants in trays of 96 and leave two spots open (for the PCR controls) 2.harvest 1 to 2 young and green leaves (1cm2 /plant, at rosette stage if possible). Use 96 well plates (1 or 2ml, E&K, pol[阅读全文]

摘要:Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5, 250 mM NaCl, 25 mM EDTA, 0.5% SDS Extraction Buffer1 M Tris-HCl pH 7.510.00 ml5 M NaCl2.50 ml0.5 M EDTA2.50 ml20% SDS1.25 mlH2O33.75 mlTotal50.00 [阅读全文]