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Methods?for?DNA?sequencing
词条创建者:admin创建时间:12-09 23:24
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摘要:Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts and the relative deoxy/dideoxynucleotide ra[阅读全文]

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Method to look for novel methylated gene-DNA Methylation
词条创建者:admin创建时间:12-09 23:16
标签: gene-DNA Methylation 甲基化

摘要:Hi. I am a new member of this forum. Currently I am trying to look for some novel methylated gene in NPC cell line. What method should I start with? Pls help. Thank you. -CHOO-------------------------[阅读全文]

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词条创建者:admin创建时间:12-09 23:14
标签: DNA分离

摘要:(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu)A.Large scale double-stranded DNA isolationThe method used for the isola[阅读全文]

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METHOD for Western Blots
词条创建者:admin创建时间:12-09 22:09
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摘要:1.While your SDS-PAGE gel is running,make your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikha,top shelf to the right side--looks lik[阅读全文]

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Methods for DNA sequencing
词条创建者:admin创建时间:12-08 23:11
标签: DNA sequencing Methods 测序 方法

摘要:A. Bst-catalyzed radiolabeled DNA sequencingBst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts[阅读全文]

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