摘要:Comments: pSV281 RPA70AB Constructed by L.Mizoue; May '02.This plasmid encodes RPA 70 subunit residues 181-422 as a fusion protein with an NT 6His tag.The tag can be cleaved off with TEV protease (rec[阅读全文]

摘要:Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct).i.Pick a single colony from freshly transformed cells (B[阅读全文]

摘要:Materials •Suspension culture of fibroblast cells (1 liter)•35 mM Tris-HCl,pH 7.4,140 mM NaCl (TBS buffer)•10 mM Tris-HCl,pH 7.5,10 mM KCl,and 1.5 mM magnesium acetate (TBS-M)•10X [阅读全文]

摘要:DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages which cause charged colloid particles to move toward[阅读全文]

摘要:蛋白质分离纯化是用生物工程下游技术从混合物之当中分离纯化出所需要得目的蛋白质的方法。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:1. Reagent and Materials (1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01) (2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O[阅读全文]