摘要:Sjogren-Larsson氏综合征又称鱼鳞癣样红皮病、痉挛性两侧瘫，为一独立疾病。 【病因和机理】 未明，为常见染色体隐性遗传性疾病。可能为免疫异常引起的中枢神经脱髓鞘病变。 【临床表现】 特征为[阅读全文]

摘要:Shy-Drager氏综合征又称Shy-Drager氏直立性低血压综合征，是指无明确病因和基础疾病的“特发性直立性低血压”。 【病因和机理】 病因未明。Shy强调本病是神经系统的原发疾病，可能为一种原[阅读全文]

摘要:Schwartz-Jampel氏综合征又称软骨营养障碍性肌强直。 【病因和机理】 病因未明。病人尿液中硫酸软骨素异常增多，故认为可能属于硫酸软骨素-4尿的一种粘多糖异常疾病。 【临床表现】 特征为睑裂[阅读全文]

摘要:SMON氏综合征SMON为Subacute myelo-optico neuropoathy（亚急性脊髓视神经痛）的缩写。 【病因和机理】 奎诺仿中毒引起脊髓和周围神经变性，有时侵犯神经，而脊髓后束和[阅读全文]

摘要:Sluder氏综合征又称面下部头痛，为一种原因未明的非典型性面痛。 【病因和机理】 未明，可能与感染、血管运动障碍、心理因素等有关。 【临床表现】 特征为一侧面部疼痛，范围不超过耳廓的高度。 女性比男[阅读全文]

摘要:基本原理是：硝酸纤维膜或尼龙滤膜对单链DNA的吸附能力很强，当电泳后凝胶经过DNA变性处理，覆以上述滤膜，再于其上方压上多层干燥的吸水纸，借助它对深盐溶液的上吸作用，凝胶上的单链DNA将转移到滤膜上。[阅读全文]

摘要:Salmon Sperm DNA1.Dissolve 1 g salmon sperm DNA in 100 ml H2 O. 2.Autoclave (20 minutes) and aliquot in 1 ml/tube 3.Store at -20℃. (common freezer for stock solutions) [阅读全文]

摘要:16 µl BigDye Terminator Ready Reaction Mix1 µg BAC DNA (determined from gel)10 pmol primerwater to 40 µlHeat tubes at 95℃ for 5 min., then perform 30 cycles of:95℃ x 30 sec55℃ x 10 s[阅读全文]

摘要:This protocol is intended to make shotgun libraries from BACS that are to be fully sequenced and assembled. To minimize chimeric clones, an adaptor method is used. There are simpler protocols if just [阅读全文]

摘要: 点击浏览该文件[阅读全文]

摘要:Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA s[阅读全文]

摘要:This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly).Solutions2X YT Media16 g tryptone10 g yeast extract5 g NaCl1 ml 1N NaOHup to 1 liter with Q[阅读全文]

摘要:This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species, as well as tobacco and[阅读全文]

摘要:Materials:Silica Suspension: add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2000 x g for 2 minestimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl, 10 mM Tr[阅读全文]

摘要: 点击浏览该文件[阅读全文]

摘要:Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it?thanks a million for any suggestions!-kiwi-Kiwi-I[阅读全文]

摘要:Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml[阅读全文]

摘要: Prepare a ligation mix:Ligation Mix (2x)10x ligase buffer 1.0 ml digested vector(0.1 mg/ml) 1.0 ml H2 O 6.0 ml total 8.0 ml divide ligation mix into two Eppendorf tubesLigation RxnInsert Control liga[阅读全文]

摘要:It is best to do a test ligation without insert, if the background is low, it would not be necessary to screen colonies for insert.1.colony PCR 1) Pick a colony from plate and suspend the colony in 10[阅读全文]

摘要:一、In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deionized water or TE buffer (10-35µl). 二、Add: （一）10X ligation buffer 5µl, （二）50% PEG 4000 solution (for blunt[阅读全文]