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搜索“TAG:sequencing”找到相关内容6篇,用时0.011641秒
摘要:16 µl BigDye Terminator Ready Reaction Mix1 µg BAC DNA (determined from gel)10 pmol primerwater to 40 µlHeat tubes at 95℃ for 5 min., then perform 30 cycles of:95℃ x 30 sec55℃ x 10 s[阅读全文]
摘要: 10X TBE, per literSequencing dye-----------------------------------108g Tris base80% formamide55g boric acid10mM NaOH40ml 0.5M EDTA1mM EDTA0.1% xylene cyanol10% APS0.1% bromophenol blue--------------[阅读全文]
摘要:10X TBE, per literSequencing dye-----------------------------------108g Tris base80% formamide55g boric acid10mM NaOH40ml 0.5M EDTA1mM EDTA0.1% xylene cyanol10% APS0.1% bromophenol blue---------------[阅读全文]
摘要:1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be [阅读全文]
摘要:Amberg Lab ,Upstate Medical University http://www.upstate.edu/biochem/amberg/protocols/seq_temp.html Notes on DNA : The cleaner the better.If mini DNA use phenol extracted and use the entire mini prep[阅读全文]
摘要:The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoF[阅读全文]