摘要:生物安全柜是用于实验室的主要隔离设备，可以防止有害悬浮微粒的扩散，对人员、样品和环境提供保护。生物安全柜按照NSF49号标准分类。二级生物安全柜属于全开门的通风橱，通过垂直气流来保护工作人员，空气经多层HEPA过滤器[阅读全文]

摘要:A simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+ , thereby preventing catalysis. If further manipulations of the digested DNA are to be performed, the res[阅读全文]

摘要:A claymation of the assembly of the spliceosome, and subsequent mRNA splicing." The Spliceosome Dance（剪切子的模拟动画） "[阅读全文]

摘要:The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced[阅读全文]

摘要:The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced[阅读全文]

摘要:pSico Oligo Design Click on the Mac OSX program pSicoOligomaker 1.5 to select and design oligos for pSico and plentilox3.7.To operate it, simply paste your sequence in the "sequence" window, give it a[阅读全文]

摘要:Overview We design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse Refseq transcript. The algorithm applies a set of [阅读全文]

摘要:Topics　　• What is the Polymerase Chain Reaction?　　• History and (pre-history) of PCR　　• How PCR works　　• Optimising PCR　　• Fidelity, errors and cloning　　• PCR primer desi[阅读全文]

摘要:SYBR Green Ⅰ荧光染料：是一种只与双链DNA小沟结合的染料，当它与DNA双链结合时，发出荧光；从DNA 双链上释放出来时，荧光信号急剧减弱。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。在PCR 反应体系中，加[阅读全文]

摘要:跑凝胶电泳，然后将蛋白质从凝胶转移到硝酸纤维素膜中再用考马斯蓝将凝胶染色，对比几组结果。[阅读全文]

摘要:Tandem mass spectrometry (MS/MS) now plays a very important role in protein identification due to its fastness and its high sensitivity.The derivation of the peptide sequence from its MS/MS spectrum i[阅读全文]

摘要:Rare event analysis in flow cytometry is nothing new. Why the statistics involved have been overlooked.本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:INTRODUCTIONMeasurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts i[阅读全文]

摘要:ECACC and Human Genetic ResearchThe European Collection of Cell Cultures (ECACC) was first established within CAMR, Salisbury, UK in 1984 and has grown to be one of the largest animal cell culture col[阅读全文]

摘要:In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. Over the last t[阅读全文]

摘要:信号传导(cell signalling) 是细胞通讯的基本概念，强调信号的产生、分泌与传送，即信号分子从合成的细胞中释放出来，然后进行传递。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]