摘要:Preparation of Frozen Competent E. coli1. For TG1 or DH5a cells, start with a colony from a minimal plate to ensure the presence of the F ' episome. Grow in 5 mls LB o/n (see note on medium below). In[阅读全文]

摘要:Kitto Lab, The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/electroporation.html This procedure prepares glycerol stock cultures of bacteria f[阅读全文]

摘要:Transformation DNA fragments (or plasmid DNA) into competent E. coli * Caution: Use aerosol protecting tips if selection of transformed cells is not based on X-gal strategy.1.Remove competent cells (E[阅读全文]

摘要:Donis-Keller Lab Manual, Department of Genetics in Washington University School of Medicinehttp://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid08.htmlPurpose: To utilize competent E. coli bacteria to rep[阅读全文]

摘要:Preparation of electroporation cells1. Prepare an overnight of NM522 in minimal medium.2. Inoculate 1L LB with 10ml (1/100th vol) of the overnight and grow to A600 = 0.5- 1.0.3. Pellet cells 5krpm, 15[阅读全文]