摘要:1. 细菌离心后，加入溶液I蜗旋振荡时，发现菌体呈絮状不易混匀，或成细砂样。—— 很可能是细菌发生溶菌，可减少培养时间、降低培养温度试试看，通常降低培养温度 会使细菌生长更加稳定；同时也可以试试平板培养 ，[阅读全文]

摘要:Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.* The 10x PCR buffer contains: 500 mM KCl; 100 mM Tris-HCl (pH 8.3); 15 mM [阅读全文]

摘要:The following tips can be used to overcome the most common problems encountered during Western blotting. Smeared Pattern or Distorted Bands Uneven contact between gel and membrane: cassettes used shou[阅读全文]