按字母顺序浏览:A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 其他

搜索“TAG:D”找到相关内容283篇,用时0.016191秒

词条| 搜标签
DEPHOSPHORYLATION?OF?LINEARIZED?DNA
词条创建者:admin创建时间:12-09 23:25
标签:

摘要:ALKALINE PHOSPATASE(CIP) DIGESTIONDigestion of protruding 5'-ends1. Precipitate digested DNA and resuspend in a 100ml of 1X CIP Buffer.2. Add 1U CIP for 100pmol 5'-ends.2g linearized 5kb plasmid &Arin[阅读全文]

编辑:0次| 浏览:3044次
词条创建者:admin创建时间:12-09 23:25
标签:

摘要:(一)质粒DNA 的提取及鉴定1.收获细菌(1)将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中,接入一单菌落,于37℃剧烈振荡培养过夜。(2)将1.5ml培养物倒入1.5ml离心管中,用台式离心机于4℃以12000g离心5min,[阅读全文]

编辑:0次| 浏览:2848次
词条创建者:admin创建时间:12-09 23:25
标签:

摘要: 限制性内切酶可特异地结合于一段被称为限制酶识别序列的DNA 序列位点上并在此切割双链DNA 。绝大多数限制性内切酶识别长度为4、5或6个核苷酸且呈二重对称的特异序列,切割位点相对于二重对称轴的位置因酶而异。一些酶恰[阅读全文]

编辑:0次| 浏览:3558次
DNase?I?Footprinting
词条创建者:admin创建时间:12-09 23:24
标签:

摘要: An end labeled DNA probe is incubated with a purified DNA-binding factor or with a protein extract. The unprotected DNA is then digested with DNase I such that on average, every DNA molecule is cut o[阅读全文]

编辑:0次| 浏览:2214次
DnaseI Footprinting
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:Protocol:Combine an appropriate concentration of probe that has been labeled on one strand (100-150 fmol probe has worked so far) with the appropriate concentration of protein in a total volume of 50&[阅读全文]

编辑:0次| 浏览:2131次
DNA Recovery With Low Melt Agarose
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:Recovery of DNA from Low Melting Point Agarose Gels1.Run digestion products on 0.7% LMP agarose gel in 1X TBE (it's nice to have at least 1ug of the fragment you want). LMP agarose is fragile; pour ge[阅读全文]

编辑:0次| 浏览:2147次
Deproteination?using?phenol/chloroform
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. Th[阅读全文]

编辑:0次| 浏览:1987次
DNA?Extraction?from?Paraffin?Section
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:点击浏览该文件 [阅读全文]

编辑:0次| 浏览:1641次
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:第一节 概 述 一. DNA的限制性内切酶酶切分析 限制性内切酶能特异地结合于一段被称为限制性酶识别序列的DNA序列之内或其附近的特异位点上,并切割双链DNA。它可分为三类:Ⅰ类和Ⅲ类酶在同一蛋白质分子中兼有切割和修饰([阅读全文]

编辑:0次| 浏览:1531次
DNA?Partial?Digestion
词条创建者:admin创建时间:12-09 23:24
标签:

摘要: 点击浏览该文件 [阅读全文]

编辑:0次| 浏览:1510次
DNaseI?Footprintint
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:DNaseI FootprintintSolutions10X Binding Buffer200 mM Tris 8.0 200 m l 1M Tris pH 8.0500 mM NaCl 100 m l 5M NaCl10 mM EDTA 20 m l 0.5 M EDTA pH 8.0680 m l Qstore at room temperatureDNaseI Dilution Buff[阅读全文]

编辑:0次| 浏览:1788次
DNA FRAGMENT PURIFICATION W/ GLASS WOOL
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:Glass wool method: Run TAE agarose gel and cut the appropriate band out with a clean razor blade. Poke a small hole with hot needle on the bottom of an eppendorf tube, and jam the hole with a little o[阅读全文]

编辑:0次| 浏览:1822次
DNA?Fragment?Purification
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:TE solution 10 mM Tris (pH to 7.5) 1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose gel piece containing the desired DNA fragmentSupplies: 1.Micropipetter and tips 2.Microcentrifuge and tubes 3.Spatu[阅读全文]

编辑:0次| 浏览:1538次
DNA?Plasmid?Maxiprep?Protocol
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampicillin or appropriate antibiotic. Shake at 250 RPM overnight.2. Centrifuge cells in a Sovall GSA (250 ml)or SLA-3000 (50[阅读全文]

编辑:0次| 浏览:3436次
DNA?Preparation?from?Blood
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:点击浏览该文件[阅读全文]

编辑:0次| 浏览:1548次
DNA?Extraction
词条创建者:admin创建时间:12-09 23:23
标签:

摘要:This is a modification of a salting out procedure as described by Miller et al., (1988), evaluated at the DNA Laboratory, Medical School, Malta. When analysed by spectrophotometry >95% of extracted ge[阅读全文]

编辑:0次| 浏览:1909次
DNA fingerprinting - agarose gel
词条创建者:admin创建时间:12-09 23:23
标签:

摘要:Restriction digests consist of:15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digestions in 96 well plates. Incubate at 37℃ [阅读全文]

编辑:0次| 浏览:1556次
DNA?Sequencing
词条创建者:admin创建时间:12-09 23:23
标签:

摘要:Pouring the Gel Outline: Pouring this big & thin 6% acrylamide gel ("Mother of all gels") is quite a challenge and probably the reason why smart whimps by them ready to use.Supplies & Equipment: water[阅读全文]

编辑:0次| 浏览:1738次
词条创建者:admin创建时间:12-09 23:23
标签:

摘要:点击浏览该文件[阅读全文]

编辑:0次| 浏览:1960次
DNA?From?Whole?Blood?for?PCR
词条创建者:admin创建时间:12-09 23:23
标签:

摘要:1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube. Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA. Mix immediat[阅读全文]

编辑:0次| 浏览:1606次
 共283条 ‹‹12345678910››... 15

更多详情,尽在互动百科
更多详情,尽在谷歌搜索