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搜索“TAG:D”找到相关内容283篇,用时0.023539秒

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DNA extraction from blood-Molecular Biology
词条创建者:admin创建时间:12-09 23:18
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摘要:I am trying to extract DNA from mammalian bloodbut while doing the phenol extractionlot of Gelatinous material in aqeous phase creating problem in its separation.Can somebody tell me how to minimise i[阅读全文]

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词条创建者:admin创建时间:12-09 23:18
标签: DNA重组 常用酶

摘要:限制性核酸内切酶 :识别DNA特定序列,切断DNA链 ;DNA聚合酶Ⅰ: 或Klenow1、缺口平移制作标记DNA探针; 2、合成cDNA的第二链;3、填补双链DNA3’凹端;4、DNA序列分析耐热DNA聚合酶(Taq DNA聚合酶等) 聚合酶链反应(PCR);D[阅读全文]

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词条创建者:admin创建时间:12-09 23:18
标签: 内切酶 酶切

摘要:限制性内切酶能特异地结合于一段被称为限制性酶识别序列的DNA序列之内或其附近的特异位点上,并切割双链DNA。它可分为三类:Ⅰ类和Ⅲ类酶在同一蛋白质分子中兼有切割和修饰(甲基化)作用且依赖于ATP的存在。Ⅰ类酶结合于识别[阅读全文]

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词条创建者:admin创建时间:12-09 23:18
标签: DNA重组

摘要:TaKaRa的内切酶和NEB的内切酶哪个更好一些? 参考见解: TaKaRa的内切酶、NEB的内切酶两个公司的酶的品质都非常好。NEB公司的酶的活性很高,切出两条小带可能是因为出现星活性,可以试试把酶量减半。NEB的酶很多都是克隆的,[阅读全文]

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词条创建者:admin创建时间:12-09 23:18
标签: DNA分离

摘要:Table of Contents10X TBE 40% Acrylamide Stock Alkaline lysis solution 10X TBE:216 g Tris base 110 g boric acid 16.6 g EDTA Add water to 2 liters.40% Acrylamide/Bisacrylamide (40% A&B):38 g Acrylamide [阅读全文]

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DNA extraction from blister rust aeciospores and urediniospores using dry grindi
词条创建者:admin创建时间:12-09 23:18
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摘要:Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Molecular Pathologist,U.S.D.A. Forest Service, North Central Forest Experiment Station,Forestry Sciences Laboratory, 5985 Hw[阅读全文]

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DNA?Extraction?from?Archival?Formali
词条创建者:admin创建时间:12-09 23:17
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摘要:DNA Extraction from Archival Formalin-fixed, Paraffin-embed allowfullscreen='true'ded Tissue SectionsAuthor: Shi et al. Source: Contributed by APostodoc Abstract: Describes two methods of extracting D[阅读全文]

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词条创建者:admin创建时间:12-09 23:17
标签: DNA分子克隆

摘要:在体外将DNA分子片段与载体DNA片段连接,转入细胞获得大量拷贝的过程中DNA分子克隆(或基因克隆)。其基本步骤包括:制备目的基因→将目的基因与载体用限制性内切酶切割和连接,制成DNA重组→导入宿主细胞→筛[阅读全文]

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DNA isolation from Avian tissue-Molecular Biology
词条创建者:admin创建时间:12-09 23:17
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摘要: I am working with birds of prey....i am looking for a simple method for isolating genomic DNA from avian breast muscle....I have found protocols for mammalian tissue...will these work fine? also i ha[阅读全文]

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词条创建者:admin创建时间:12-09 23:17
标签: DNA计算分析

摘要:Sequence Analysis . Automated (Semi-Automated) Sequencers generate a four-color chromatograms on the results of the sequencing gel, as well as a text file of sequence data. The sequencer can not verif[阅读全文]

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DNA?Purification?from?Agarose?Gels
词条创建者:admin创建时间:12-09 23:17
标签: Purification Agarose

摘要:1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel with a razor blade above and below the bands of inter[阅读全文]

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词条创建者:admin创建时间:12-09 23:17
标签: DNA片段 纯化回收

摘要:载体及目的DNA 片断经酶切后,如果无多余的片断(如载体单切)可以直接酚/氯仿抽提后乙醇沉淀回收直接连接用。但多数还有多余片断(如载体双酶切后有插入片断但要回收空载体,多个目的DNA片断选择其一克隆),必须电泳分离后[阅读全文]

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Dialysis?(using?dialysis?tubing)
词条创建者:admin创建时间:12-09 23:17
标签: Dialysis

摘要:Molecularpourous membrane tubing is used for desalting, protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see g[阅读全文]

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DNA Purification-Modified CTAB Procedure
词条创建者:admin创建时间:12-09 23:17
标签: Purification-Modified Procedure

摘要:This protocol originated in Murray and Thompson (1980) and then was modified by Richard Jorgensen and finally published in Wagner et. al 1987.1.Start with about 10 grams fresh needles. 2.Chop into sho[阅读全文]

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DNA?Extraction?Methods
词条创建者:admin创建时间:12-09 23:16
标签: DNA Extraction

摘要:Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand fliesin 0.5 ml lysis buffer (50 mM NaCl , 10 mM EDTA, 50 mM Tris-HCl pH 8). 2.Incubate The sand fly homogenateswith 100 ng/ml R[阅读全文]

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Differences between MSP and BSP-DNA Methylation, Histon
词条创建者:admin创建时间:12-09 23:16
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摘要:Hello everyone I'm new here and I was wondering if I could get some assistance.In my research of the literature I thought the differences between the two methods were based on the proximity of the pri[阅读全文]

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词条创建者:admin创建时间:12-09 23:16
标签: DNA粗提 DNA鉴定

摘要:1、实验原理鸟类血液中红细胞有细胞核,细胞核内DNA与蛋白质结合成染色质。利用DNA在0.12-0.14mol/L的NaCl溶液中溶解度最低,可使溶液中的DNA形成沉淀析出,通过过滤,得到初提取的DNA。再利用DNA不溶于酒精溶液,而细胞中其[阅读全文]

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DNA concentration-Molecular Biology
词条创建者:admin创建时间:12-09 23:16
标签: concentration-Molecular

摘要:Does somebody know how to increase DNA concentration from a too weakly concentrated DNA sample obtained with a Qiagen Extraction Kit (the elution buffer was ddH2O) ?? -BATY----------------------------[阅读全文]

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词条创建者:admin创建时间:12-09 23:16
标签: DNA转化

摘要:实验原理:体外通过基因工程手段所构建的含目的基因的重组质粒,选用转化和筛选技术, 可获得含重组的阳性克隆。在此阳性克隆中,DNA可在生物体系中大量扩增,繁殖, 保存以及表达目的基因的产物,这是PCR体外扩增DNA所不能[阅读全文]

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DNA sequencing (dye terminator)
词条创建者:admin创建时间:12-09 23:16
标签: sequencing

摘要:Hahn Lab,The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/BigDye Protocol.pdf [阅读全文]

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