摘要:甲基化研究对于基因的调控和肿瘤的发生有非常密切的关系。在一般正常的细胞中，基因调控区CpG岛处于非甲基化的状态。而当细胞发生癌变后，这些CG区域往往呈现甲基化状态。英国医学刊物《the Lancet》报道奥地利因斯布鲁克医[阅读全文]

摘要:实验目的 要获得表达的基因AKP，必须构建重组子。 我们通过以下的方法构建重组DNA，转入受体菌，就可以表达出目的基因。 之所以重组子要进入细胞，是因为大肠杆菌中有修饰的机制，可以在转入表达载体时不被降解，高效表达。 [阅读全文]

摘要:Hi....I need a protocol for extracting DNA from hair/roots...(specifically horse)...and any additional information such asstorage pr℃edure/timing/etc....thanks.... -tree-------------------------------[阅读全文]

摘要: DNA微阵列技术（microarray）指在固体表面（玻璃片或尼龙膜）上固定成千上万DNA克隆片段，人工合成的寡核苷酸片段，用荧光或其他标记的mRNA,cDNA或基因组DNA探针进行杂交，从而同时快速检测多个基因表达状况或发现新基因，[阅读全文]

摘要:一、个体识别 个体识别以同一认定理论为指导原则。通过对物证检材 的遗传标记作出科学鉴定，依据个体特征来判断前后两次或多次出现的物证检材是否同属一个个体的认识过程。第一次出现的往往是与案件事实有联系，并且是在[阅读全文]

摘要:Q: 怎样对合成DNA制品进行定量?A: DNA的量与260 nm处的吸光度值（OD值）成正比，因此使用紫外分光光度计定量是最科学的。1个OD值的合成DNA的重量约为33 mg。Q: 怎样理解测定的OD值?A: 进行OD值测定时，分光光度计上显示的[阅读全文]

摘要:DNA分子的体外连接就是在一定条件下， 由DNA连接酶催化两个双链DNA片段组邻的５’端磷酸与３’端羟基之间形成磷酸酸脂键的生物化学过程， DNA分子的连接是在酶切反应获得同种酶互补序列基础上进行的。带有相同[阅读全文]

摘要:Chapter 4 replicationDNA replicationDNA damage and repairReverse transcriptionRNA replicationDNA复制的基本特点半保留复制(self-conservative replication)复制子(replicon)半保留复制1958年,Meselson 和Stahl[阅读全文]

摘要:This is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologist, and areas of interest are outlined. Tissue blocks [阅读全文]

摘要:常见问题具体情况可能的原因处理办法备注样品准备问题菌培养不好或失败抗性不对或菌已死核对抗性，尽可能提供载体信息。 或菌培养条件特殊重新提供菌液，或提供2μg纯化质粒。质粒提不出质粒拷贝数极低或客户自己采取[阅读全文]

摘要:DNA的克隆是指在体外将含有目的基因或其它有意义的DNA片段同能够自我复制的载体DNA连接，然后将其转入宿主细胞或受体生物进行表达或进一步研究的分子操作的过程，因此DNA克隆又称分子克隆，基因操作或重组DNA技术。DNA克[阅读全文]

摘要:DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA.The most popular method for doing this is called the dideoxy method.The equipment and supplies:·Ste[阅读全文]

摘要:1.Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc,pH 7.0 300 ml 1 M NaOAC,pH 7.01 mM EDTA 2 ml 500 mM EDTA,pH 8.0698 ml ddH202.Incubate at 22℃ for 30 min.Transfer gel sli[阅读全文]

摘要:This protocol was written by Jean-Pierre Issa,based on Adams et al.* Kam-Wing Jair has made some useful shortcuts that work well if you are careful.Here is .This assay can be used to measure activity [阅读全文]

摘要:摘要： DNA 甲基化是表观遗传学(Epigenetics)的重要组成部分，在维持正常细胞功能、遗传印记、胚胎发育以及人类肿瘤发生中起着重要作用，是目前新的研究热点之一。随着对甲基化研究的不断深入，各种各样甲基化检测方法被开[阅读全文]

摘要: MATERIALS:The DEAE paper can be used as supplied by Schleicher and Schuell, however the binding capacity can be increased by washing the paper in 10mM EDTA, pH 7.5 for 10 minutes, 0.5M NaOH for 5 min[阅读全文]

摘要:Section of Cancer Genomics,Genetics Branch,NCINational Institutes of HealthReagentsChloroformMallinckrodt,cat.4440EDTA,0.5 MEthanol,100%Ethanol,70%Isoamyl AlcoholSigma,cat.I-3643PhenolGibco BRL,Cat.15[阅读全文]

摘要:DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group,catalyzed by DNA methyltransferase (DNMT),to the 5-c[阅读全文]

摘要:Lab of Dr. Mark Barton Frank, Oklahoma Medical Research Foundation http://omrf.ouhsc.edu/~frank/LIGATION.html [阅读全文]

摘要:1.Prepare or obtain Buffered phenol,pH 8.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored.2.Combine DNA sample with an equal volume of[阅读全文]