摘要:Deproteination using phenol/chloroform (Maniatis et al.,1982)'Phenol' as used is Analar grade.Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%,and equilibrated [阅读全文]

摘要:1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Locate bands with a hand-held long-wave UV lamp.2.Slice the gel with a razor blade above and below the bands of interest[阅读全文]

摘要:Materials:1.TE solutiono10 mM Tris (pH to 7.5)o1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose gel piece containing the desired DNA fragmentSupplies:1.Micropipetter and tips2.Microcentrifuge and tub[阅读全文]

摘要:A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves(See also1)Take one medium sized leaf or half a large leaf [阅读全文]

摘要:1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapillary tube.Expel blood immediately into a 1.5 ml microfuge tube containing 20 µl of 10 mM EDTA.Mix immediatel[阅读全文]

摘要:[原理]利用DNA 连接酶把载体 DNA 和要克隆的目的 DNA 片段连接在一起，成为一个完整的重组分子，这就是分子克隆中常说的连接反应。当载体 DNA 和外源 DNA 末端都是由一种产生粘性末端的内切酶切割产生的话（同尾酶也可以[阅读全文]

摘要:1.没有回收到 DNA 片段如在洗脱后,发现洗脱液中没有 DNA 片段,请检查是否按瓶身标签,在洗涤液中加入了无水乙醇.2.提取率低(1)融胶液为酸性缓冲液,如融胶后,其pH 值升高将影响提取得率.请加入0.1 倍体积的3M乙酸钾(pH[阅读全文]

摘要:一.实验目的 1.掌握DNA指纹图谱技术的概念、原理和基本操作过程2.学习DNA的限制性酶切的基本技术3.掌握琼脂糖凝胶电泳的基本操作技术，学习利用琼脂糖凝胶电泳测定DNA片段的长度，并能对实验结果进行分析。二.实验原理19[阅读全文]

摘要:DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting （使用前的重要注释） 1、Take time to familiarize yourself with th[阅读全文]

摘要:实验目的 将复杂的细胞分子混合物加入有机溶媒萃取以除去蛋白质及其它成分，就可以纯化DNA。一般常用酚及氯仿(phenol/chloroform)可使蛋白质变性(denaturaion)的特性来进行萃取的步骤，DNA和RNA不溶于有机溶媒中，而溶于[阅读全文]

摘要:Jacks Lab,Center for cancer research,MIT 1.Each tail should be in a clean eppendorf tube.2.Add 500µl of tail lysis buffer containing Proteinase K (PK)to each tube.3.Incubate tail samples in 5060[阅读全文]

摘要:DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to 10 kb the agarose purification is ideal.For smaller fragments (20 bp to 400 bp)the acrylamide purification is preferred[阅读全文]

摘要:[原理]DNA 限制性内切酶消化是基因分析中的关键步骤。内切酶是最关键的工具酶。限制性内切酶是一类具有严格识别位点,并在识别位点内或附近切割双链DNA 的脱氧核糖核酸酶。酶单位规定为:在最适反应条件下 1 小时完全消化[阅读全文]

摘要:CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES(see also DNA RNA double isolation procedure if both DNA and RNA are needed)Reagents ne[阅读全文]

摘要:Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnormal [阅读全文]

摘要:Sugden lab,McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School DnaseI Footprinting(Based upon the protocol from the Promega Core Footprinting Kit)5x Binding Buffer ([阅读全文]

摘要:摘要： DNA甲基化是表观遗传学(Epigenetics)的重要组成部分，在维持正常细胞功能、遗传印记、胚胎发育以及人类肿瘤发生中起着重要作用，是目前新的研究热点之一。随着对甲基化研究的不断深入，各种各样甲基化检测方法被开发[阅读全文]

摘要:DNA序列测定分手工测序和自动测序，手工测序包括Sanger双脱氧链终止法和Maxam-Gilbert化学降解法。自动化测序实际上已成为当今DNA序列分析的主流。美国PE ABI公司已生产出373型、377型、310型、3700和3100型等DNA测序仪，其[阅读全文]

摘要:What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively charged molecule, and is moved by electric curre[阅读全文]

摘要:带电荷的物质在电场中的趋向运动称为电泳。电泳的种类多，应用非常广泛，它已成为分子生物学技术中分离生物大分子的重要手段。琼脂糖凝胶电泳由于其操作简单、快速、灵敏等优点，已成为分离和鉴定核酸的常用方法。实验目的：[阅读全文]