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搜索“TAG:I”找到相关内容71篇,用时0.013890秒
摘要:应用已知碱基序列并带有标记物的核酸探针与组织、细胞中待检测的核酸按碱基配对的原则进行特异性结合而形成杂交体,然后再应用与标记物相应的检测系统,通过组织化学或免疫组织化学方法在被检测的核酸原位形成带颜色的[阅读全文]
摘要:RNA for S1 or PE analysis must be free of chromosomal DNA.This can be accomplished by extraction of the RNA with hot phenol, but phenol can, especially if its pH is acidic, cause the specific loss of [阅读全文]
摘要:In vitro transcription with yeast nuclear extract Steve HahnLast Modified Fri, Apr 25, 2003Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and [阅读全文]
摘要:in vitro TranscriptionReaction1 µl 10X Transcription Buffer (Ambion) 1 µl 10X NTPs (4 mM ATP, CTP, 1 mM GTP, UTP) 2 µl 10 mM GpppG cap (Pharmacia) 2 µl a[32P]-UTP (NEN) 800 Ci/[阅读全文]
摘要:The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3.A 5-primer with[阅读全文]
摘要:1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X transcription buffer ( stratagene )1 ml 750 mM DTT1 ml Rnasin (RNase inhibitor; 15U/ml)1 ml 10 mM ATP (pH 7)1 ml 10 mM CTP [阅读全文]
摘要:Steve HahnLast Modified Fri, Apr 25, 2003Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before use to eliminate [阅读全文]
摘要:N.B: Gloves should be worn at all times during preparation of in vitro RNA. All solutions should be RNase-free i.e. made with DEPC water if home-made or bought specifically to use for RNA work. You wi[阅读全文]
摘要: OverviewWith this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are use[阅读全文]
摘要:In vitro transcription with yeast nuclear extract Steve HahnWear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol befor[阅读全文]
摘要:For more information please visit our homepage www.intelligent-siRNA.com Intelligent siRNA [阅读全文]
摘要: Analysis of the interaction of proteins with either DNA or RNA sequences by in vivo footprinting involves two steps: (i) the in situ modification of nucleic acids by the footprinting reagent and (ii[阅读全文]
摘要:对CDC SARS的实验室反应的几个阶段的纪录,得出了一些结论。[阅读全文]
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摘要:由于SPF级动物需要投入大量的经费和时间,投资者必须保护这些动物免受污染。关于隔离水平,存在着以下几种不同的水平:房间水平,弹性膜隔离和笼具水平的隔离。其中,笼具水平的隔离是最具有可塑性和实用性的装置,它由一个[阅读全文]
摘要:sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 min wash 2-4x with sterile H2O for 1 min plating and growth[阅读全文]