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摘要:Hi,guys, :I have a problem about mutagenesis. I want to mutate 2 base pairs in plasmid,and use stratagenne mutagenesis protocol.Design 2 oligos as primers.After I run PCR, I only see one band less tha[阅读全文]
摘要:I'm looking for a methode to demethyle DNA in vivo , during more than 5 days . All I've read about 5-aza-CdT is for maximum 5 days. Why ?Is there another drug and if yes, how does it work ?thanksElea-[阅读全文]
摘要:Alkaline lysis miniprep1. Grow bacteria overnight in 37℃shaking incubator, with lids very loose and taped on. I normally use 5 ml of liquid medium in a 50ml conical bottom tube. Be sure to include the[阅读全文]
摘要:Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2. Allow the silica to settle for 2h.3. Discard the supernatant containing fine particulate matter.4. Repeat steps 2 and 3[阅读全文]
摘要:does anyone have a reasonably successful protocol for isolating dna from bones---bones that are highly compromised in integrity---i.e.,really bad shape?I've tried and am well seasoned in the tradition[阅读全文]
摘要:hi,everybody.i want extract DNA from cryopreserved clotted human blood.the smaple has been storaged 5 years in -20 degree.who is an expert on this topic .i need your help.i need the details.my email i[阅读全文]
摘要:Use from 0.01 - 0.1 gram plant material.Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.Transfer the ground tissue to a eppendorf tube.Add 1 ml extra[阅读全文]
摘要:1.Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2.Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit)at 55E C for 5 min or until the gel dissolves.3.Add[阅读全文]
摘要:Hi,I'm starting to work whit 5' cytidine on plants.I want to know if it's possible to prepare a stock solution to store it at 20℃ In that conditions,the drug lose the desmetilant capacity?I've wrote t[阅读全文]
摘要:Smith Lab,Division of Biological Sciences,University of Missouri http://www.biosci.missouri.edu/smithgp/PhageDisplayWebsite/AmplifyingLibrary.docThis section describes how to amplify a library with mi[阅读全文]
摘要:This section describes how to amplify a library with minimal danger of substantially reducing its diversity.Amplification is accomplished by infecting fresh cells with a portion of the library,growing[阅读全文]
摘要:Agarose gel electrophoresis (2) is employed to check the progression of a restriction enzyme digestion, to quickly determine the yield and purity of a DNA isolation or PCR reaction, and to size fracti[阅读全文]
摘要:Analysis of genome-wide association data[阅读全文]
摘要:This is the preferred method for yeast RNA preparation use Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fresh YPD media and grow cells at 30 degrees overnigh[阅读全文]
摘要:A novel approach for evaluating the efficiency of siRNAs on protein levels in cultured cells. Wu W, Hodges E, Redelius J, Hoog C. Nucleic Acids Res. 2004 Jan 22;32(2):E17. Center for Genomics and Bioi[阅读全文]
摘要:Gregory Hannon, Cold spring harbor labOur overall approach is to use an RNA polymerase III promoter to driveexpression of encoded short hairpin RNA (shRNA). For this purpose we use the humanU6 snRNA p[阅读全文]
摘要:AtlasTM cDNA表达距阵(Expression Array)同时对588种/1176种关键基因做全景表达图谱分析同时对大量已知基因的表达进行大规模高通量(High-htroughput)分析检测关键基因在细胞关键功能中的角色只需简单的杂交步骤就在[阅读全文]
摘要:• 以96孔板方式,从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA• 处理 96 个样本,只需不到60 分钟• 从同一样本中纯化高品质的基因组DNA和总RNA• 96孔板方式,高效的操作流程• 高度标准[阅读全文]
摘要:1.Two rounds of DNA synthesis to incorporate fixed sequencesPrepare the following:Reaction tube containing the following:7ml DNA to be amplified and labelled2ml 5X T7 Sequenase Buffer1ml 50mM Pimer 1 [阅读全文]