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摘要:1、打开电源、稳压器、打印机、工作站、主机。2、工作站选择MS-DOS方式下操作,进入AFS系统。程序自检,全部出现PASS,同时听到“咔嗒”响声。3、按正确方向插上A、B道灯,用调光器调整光路,打开自动进样系统。4、按键,选定[阅读全文]
摘要:1、新建文件菜单File → New,Assay选择Absolute Quantification(实时定量模式),打开一个空白的96孔板文件。2、探针设置双击任意一个孔,打开Well Inspector窗口。该窗口也可以从菜单View → Well Inspector打开。3[阅读全文]
摘要:1【产品名称】 串联飞行质谱仪(蛋白质组分析系统) 2【产品型号】 ABI-028_TY8412 3【产品规格】 4700 4【产品产地】 美国应用生物系统公司(ABI) 5【产品详细描述】4700TOF/TOF蛋白质组分析系统是全球第一台TOF/TOF 串联飞[阅读全文]
摘要:药物研发、个性化用药研究是基因芯片技术在药物开发中应用最为广泛的一个领域。在药物靶点发现、药物作用机制、药物毒理评估研究中所使用的生物芯片主要是检测RNA水平的表达谱芯片;在个性化用药研究中,主要从人群的SNP入[阅读全文]
摘要:1、色谱柱长时间不用,存放时,柱内应充满溶剂,两端封死(如ACN适于反相色谱柱,正相色谱柱用相应的有机相)。2、对于手动进样器,当使用缓冲溶液时,要用水冲洗进样口,同时搬动进样阀数次,每次数毫升。3、流动相使用前必须过滤,[阅读全文]
摘要:(一)开机:(二)数据采集方法编辑:(三)数据分析方法编辑:(四)关机:《Agilent1100液相基本操作步骤》下载点击这里下载[阅读全文]
摘要:1、【仪器名称】:ALC-EECG多导同步心外膜电图系统。2、【仪器型号】:ALC-EECG16-32。3、【生产厂家】:上海奥尔科特生物科技有限公司。4、【检测适用范围】:应用于心肌缺血、缺氧及药物防治等研究实验。5、【仪器使用优点、缺点】:特别针对[阅读全文]
摘要:质谱法(Mass Spectrometry, MS)是利用电磁学原理,对荷电分子或亚分子裂片依其质量和电荷的比值(质荷比,m/z)进行分离和分析的方法。此视频介绍安捷伦质谱仪的原理。[阅读全文]
摘要:Acid Adjusted Swine Serum1. The before or morning of: adjust the pH of the swine serum to between 4.3 and 4.5 with 1N HCl.2. Place serum for at least 1 hour at 4°C (overnight is best).3. Centrifug[阅读全文]
摘要:annexinV-FITC(FL1 通道)+ PI(FL2通道)双染细胞,FITC阳性+PI阴性细胞百%就是早期凋亡%。一、原理磷脂酰丝氨酸(Phosphatidylserine,PS)正常位于细胞膜的内侧,但在细胞凋亡的早期,PS可从细胞膜的内侧翻转到细胞膜的表面,[阅读全文]
摘要:1. Conjugation of carrier (BSA) to peptide(1) Dissolve pure BSA 20mg/ml in 10mM phosphate, pH 7.0.(2) Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide ester succinimidyl 4- (N-maleimidomethy[阅读全文]
摘要:This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.1. Solutions(1) Affigel Blue Prewash0.1 M acetic acid 5.7 ml glacial acetic acid1.4 M NaCl 81 g [阅读全文]
摘要:FUSION 5 changes the way that lateral flow strips are developed and produced.本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:Most biological systems can be viewed in terms of interactions between receptors and epitopes. Specificity of the interaction between the receptor and epitope is determined by the amino acid sequence [阅读全文]
摘要:MaterialsPrepared slides of Ascaris megalocephala maturationMicroscopeProcedure1.Place the slide(s) of Ascaris on the microscope and focus on the eggs within the body.Unlike the grasshopper testis whe[阅读全文]
摘要:Materials from a workshop held March, 1997 in Modena, Italy. All materials reproduced with permission.Introduction A. CossarizzaWorkshop SponsorsMethods in analysis of apoptosis and cell necrosis. Z. [阅读全文]
摘要:1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml .2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 [阅读全文]
摘要:与原位活细胞在线检测仪ABER 能实时在线检测发酵罐内活细胞的浓度,密度,干重,生物量体积,电容大小,导电率大小;而不受细胞碎片,细胞团块,死细胞,发酵液泡沫,微载体颗粒等影响。适用范围: 动植物细胞,酵母,细菌藻类等种[阅读全文]
摘要:Authentication Techniques Whatever the scope of work to be carried out it is important to know that the work is being conducted using the correct reagents. This is no less important for cell cultures,[阅读全文]