摘要:General Annexin V Staining ProcedureSolutions1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a[阅读全文]

摘要:Product ListCatalog #DescriptionSize Storage Biohazard 10-103-000CMVAD169 Purified Virus1mg-70℃B 10-110-000HSV-1MacIntyre Purified Virus1mg-70℃B 10-111-000HSV-2G Purified Virus1mg-70℃B 10-112-000HTLV-[阅读全文]

摘要:1.Authentication TechniquesWhatever the scope of work to be carried out it is important to know that the work is being conducted using the correct reagents. This is no less important for cell cultures[阅读全文]

摘要:1.Cell Culture Scale-up SystemsMost tissue culture is performed on a small scale where relatively small numbers of cells are required for experiments. At this scale cells are usually grown in T flasks[阅读全文]

摘要:ACDP Advisory Committee on Dangerous Pathogens BSA Bovine Serum albumin BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhoea virus FBS Fetal bovine serum EC European Community ECACC Europe[阅读全文]

摘要:问：A549细胞到底是如何形态？我见过三角形，近乎MDCK细胞形态的，还有的呈长梭形。我感觉自己的细胞好像混进了MDCK细胞。大家帮忙诊断一下，谢谢。我培养的A549细胞答1：A549 细胞正常状态下都呈长梭形.从你提供的细胞图片看[阅读全文]

摘要:AAV-293细胞较喜欢成团生长，比较娇嫩，怕冷，传代时不要一次从二氧化碳培养箱拿出很多瓶，一次只要拿2-3瓶出来传就可以了，否则细胞很容易死掉。细胞在50-60%传代，细胞生长状态最好。用D-Hank's液配制成0。25%的胰酶消化半[阅读全文]

摘要:我养了三种贴壁的细胞，用同样的消化方法，都很好。这三种细胞是A549，ECV304，2BS。1、0.25%胰酶新鲜解冻,不需要预热。2、倒掉培养瓶中的培养基,并用预冷的D-Hank‘s洗两次（要冷，用前4度中取出）。3、加入约1-2ml的胰酶,晃[阅读全文]

摘要:Contributor: Suprya JayadevDate: November 4, 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend f[阅读全文]

摘要:问：谁有a一醋酸萘酯酶（ANAE)染色法的具体步骤，我们这里查不到，还望各位大侠不吝赐教！答：中性非特异性脂酶(α—NAE)染色[方法学] 酯酶染色的方法很多，根据与pH底物等不同作用，将常用的酯酶染色法分为两大类[阅读全文]

摘要:ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, 5-methyl cytosine, and t[阅读全文]

摘要:请下载链接：Array_Enrichment_AP_f.pdf[阅读全文]

摘要:Q:I've been getting mixed messages about the accuracy of the reads coming off the SOLiD platform. A 454 representative swore blind to me last week that only 25% of SOLiD reads are error-free (!), but [阅读全文]

摘要:FGFR-AP-FGF Binding Assay, PCR Assays for Knockout Mice, RNase Protection Assay:The bound receptor was quantified by measuring the initial rate of substrate hydrolysis at 405 nm and same amount of pro[阅读全文]

摘要:This method works well for 17.5 day mouse embryos. All stains should be made up fresh. Fix embryos in 90% ethanol for at least 1 week, longer if possible. Prior to staining remove skin, and viscera, p[阅读全文]

摘要:The aggregation method for generating chimaeras, as opposed to the microinjection technique, is useful as it does not require expensive microinjection apparatus or sophisticated manipulative skills, a[阅读全文]