摘要:The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing [阅读全文:]
摘要:The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recomb[阅读全文:]
摘要:This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown h[阅读全文:]
摘要:A. AbstractThis protocol can be used for detection of gangliosides.Spicific antibodies to gangliosides are added to cells and allowed to bind to the cell surface. The nonbound antibody is washed away [阅读全文:]
摘要:A. DAY 1 PROCEDURE??ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for[阅读全文:]
摘要:1、Q: 要测定打疫苗三次的抗体的变化，当ELISA标化后，是不是要用同一个抗原的包被浓度，同一个血清的稀释度，然后看OD值的变化？实验动物有多个，实验数据又如何处理呢？A: nanwxd网友观点：关于是不是要用同一个抗原的包被[阅读全文:]
摘要:ELISA即酶联免疫吸附试验，是一种常用的固相酶免疫测定方法。Engvall 和Perlmann于1971年最先应用该法进行了IgG定量测定，并命名为"enzyme linked immunosorbent assay (ELISA)"。ELISA的基本原理是：①使抗原（或抗体）结合[阅读全文:]
摘要:临床检验常见的标本一般包括血液（指血，静脉血），尿，粪便，脑脊液，胸腹水，前列腺液，精液，阴道分泌物等，这些标本收集的时间、方法和保存都有一定的要求。一、血液标本有些生理因素，如吸烟、进食、运动、情绪波动、妊娠、体位等均[阅读全文:]