Make up 20% DMSO in FBS. Keep on ice. (Do not put 100% DMSO on ice or it will form crystals.)Mix cells gently with 20% DMSO in FBS at a ratio of 1:1 (the final cell suspension will be 90%FBS/10% DMSO). Rapidly transfer 1 mLof cells in freezing medium to each cryovial. |
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