SNP Detection Technology

SNP detection and scoring methods are many and various. Two recurrent themes are elegant simplicity and advanced technology. An effective method recently developed by the HGBASE team is Dynamic Allele-Specific Hybridization. Currently, most procedures involve target sequence PCR amplification, a burden that limits possibilities for scale-up and automation. This is equally true for the much acclaimed miniature hybridisation array (DNA-chip) concept.Genomic level scoring of cSNPs presents another largely unrecognized hurdle due to the possible existence (usually unknown)of processed pseudogenes.

These are the articles on the technology mentioned above (in PDF):

Dynamic Allele-Specific Hybridization (Articles).pdf

Dynamic Allele-Specific Hybridization (Method).pdf

For more, you may visit the SNP Consortium's protocol page ( http://snp.cshl.org/index.html.en ), there is a lot of detailed information on the detection technology.