摘要:Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.* The 10x PCR buffer contains: 500 mM KCl; 100 mM Tris-HCl (pH 8.3); 15 mM [阅读全文]

摘要:The following tips can be used to overcome the most common problems encountered during Western blotting. Smeared Pattern or Distorted Bands Uneven contact between gel and membrane: cassettes used shou[阅读全文]