摘要:Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at RT for 3 min. Resuspend bacterial pellet in 350 µl of STET buffer. Add 25 µl of freshly prepared solution of [阅读全文]

摘要:Procedure for 100 ~ 300 mg of tissue.1. Collect fresh tissue, coleoptiles or small amount of leaf tissue.Use about three inches of a leaf blade. Preferably younger tissue. Fold leaf and place in a 2 m[阅读全文]